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Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem
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| Title: | Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem |
| Authors: | Nakamura, Yukiko Browse this author | | Hayashida, Kyoko Browse this author | | Delesalle, Victoire Browse this author | | Qiu, Yongjin Browse this author →KAKEN DB | | Omori, Ryosuke Browse this author →KAKEN DB | | Simuunza, Martin Browse this author | | Sugimoto, Chihiro Browse this author →KAKEN DB | | Namangala, Boniface Browse this author | | Yamagishi, Junya Browse this author →KAKEN DB |
| Keywords: | Trypanosoma vivax | | Trypanosoma vivax bovine trypanosomosis | | Trypanosoma vivax-like | | African animal trypanosomosis | | cathepsin L-like cysteine protease | | anemia |
| Issue Date: | 27-Jan-2021 |
| Publisher: | Frontiers Media |
| Journal Title: | Frontiers in veterinary science |
| Volume: | 8 |
| Start Page: | 599815 |
| Publisher DOI: | 10.3389/fvets.2021.599815 |
| Abstract: | We clarified the genetic diversity of Trypanosoma spp. within the Kafue ecosystem, using PCR targeting the internal transcribed spacer 1 and the cathepsin L-like cysteine protease (CatL) sequences. The overall prevalence of Trypanosoma spp. in cattle and tsetse flies was 12.65 and 26.85%, respectively. Cattle positive for Trypanosoma vivax had a significantly lower packed cell volume, suggesting that T. vivax is the dominant Trypanosoma spp. causing anemia in this area. Among the 12 operational taxonomic units (OTUs) of T. vivax CatL sequences detected, one was from a known T. vivax lineage, two OTUs were from known T. vivax-like lineages, and nine OTUs were considered novel T. vivax-like lineages. These findings support previous reports that indicated the extensive diversity of T. vivax-like lineages. The findings also indicate that combining CatL PCR with next generation sequencing is useful in assessing Trypanosoma spp. diversity, especially for T. vivax and T. vivax-like lineages. In addition, the 5.42% prevalence of Trypanosoma brucei rhodesiense found in cattle raises concern in the community and requires careful monitoring of human African trypanosomiasis. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81084 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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