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Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem

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Title: Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem
Authors: Nakamura, Yukiko Browse this author
Hayashida, Kyoko Browse this author
Delesalle, Victoire Browse this author
Qiu, Yongjin Browse this author →KAKEN DB
Omori, Ryosuke Browse this author →KAKEN DB
Simuunza, Martin Browse this author
Sugimoto, Chihiro Browse this author →KAKEN DB
Namangala, Boniface Browse this author
Yamagishi, Junya Browse this author →KAKEN DB
Keywords: Trypanosoma vivax
Trypanosoma vivax bovine trypanosomosis
Trypanosoma vivax-like
African animal trypanosomosis
cathepsin L-like cysteine protease
Issue Date: 27-Jan-2021
Publisher: Frontiers Media
Journal Title: Frontiers in veterinary science
Volume: 8
Start Page: 599815
Publisher DOI: 10.3389/fvets.2021.599815
Abstract: We clarified the genetic diversity of Trypanosoma spp. within the Kafue ecosystem, using PCR targeting the internal transcribed spacer 1 and the cathepsin L-like cysteine protease (CatL) sequences. The overall prevalence of Trypanosoma spp. in cattle and tsetse flies was 12.65 and 26.85%, respectively. Cattle positive for Trypanosoma vivax had a significantly lower packed cell volume, suggesting that T. vivax is the dominant Trypanosoma spp. causing anemia in this area. Among the 12 operational taxonomic units (OTUs) of T. vivax CatL sequences detected, one was from a known T. vivax lineage, two OTUs were from known T. vivax-like lineages, and nine OTUs were considered novel T. vivax-like lineages. These findings support previous reports that indicated the extensive diversity of T. vivax-like lineages. The findings also indicate that combining CatL PCR with next generation sequencing is useful in assessing Trypanosoma spp. diversity, especially for T. vivax and T. vivax-like lineages. In addition, the 5.42% prevalence of Trypanosoma brucei rhodesiense found in cattle raises concern in the community and requires careful monitoring of human African trypanosomiasis.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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