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Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine-producing bacterium, Morganella psychrotolerans

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Title: Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine-producing bacterium, Morganella psychrotolerans
Authors: Wang, Di Browse this author
Yamaki, Shogo Browse this author →KAKEN DB
Kawai, Yuji Browse this author →KAKEN DB
Yamazaki, Koji Browse this author →KAKEN DB
Keywords: Disinfection
Peracetic acid
Disinfectant effect
Seafood safety
Antimicrobial mechanism
Morganella psychrotolerans
Issue Date: May-2020
Publisher: Elsevier
Journal Title: LWT - Food science and technology
Volume: 126
Start Page: 109263
Publisher DOI: 10.1016/j.lwt.2020.109263
Abstract: In this study, we aimed to investigate the sanitizing effects of peracetic acid (PAA), and the underlying mechanism of its antimicrobial action against Morganella psychrotolerans. The exposure of M. psychrotolerans to 20 ppm PAA for 5 min led to its decrease below the detection level, indicating that PAA has a significant antibacterial effect against M. psychrotolerans in vitro. Moreover, the viable counts of M. psychrotolerans on saury (Cololabis saira) surface were showed a reduction of 1.40 and 2.23 log CFU/cm(2) upon treatment with 80 ppm PAA for 1 and 5 min, respectively. Further, PAA treatment delayed the growth of M. psychrotolerans on saury surface during storage at 4 degrees C. Next, the antimicrobial mechanism of PAA against M. psychrotolerans cells was investigated, and the damage to the cell membrane and cell surface upon PAA treatment was observed using scanning electron microscopy (SEM) and epifluorescence microscopy. The chromosomal DNA and the protein profiles after PAA treatment were also analyzed. Form our results, we hypothesized that the bactericidal effect of PAA treatment was mainly attributed to damage the bacterial cell membrane. These results indicate that PAA may be an efficient disinfectant against M. psychrotolerans and has applications in seafood processing and storage.
Rights: © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山﨑 浩司

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