| Title: | An African tick flavivirus forming an independent clade exhibits unique exoribonuclease-resistant RNA structures in the genomic 3 '-untranslated region |
| Authors: | Harima, Hayato Browse this author →KAKEN DB |
| Orba, Yasuko Browse this author →KAKEN DB |
| Torii, Shiho Browse this author →KAKEN DB |
| Qiu, Yongjin Browse this author →KAKEN DB |
| Kajihara, Masahiro Browse this author →KAKEN DB |
| Eto, Yoshiki Browse this author |
| Matsuta, Naoya Browse this author |
| Hang'ombe, Bernard M. Browse this author |
| Eshita, Yuki Browse this author →KAKEN DB |
| Uemura, Kentaro Browse this author |
| Matsuno, Keita Browse this author →KAKEN DB |
| Sasaki, Michihito Browse this author →KAKEN DB |
| Yoshii, Kentaro Browse this author →KAKEN DB |
| Nakao, Ryo Browse this author →KAKEN DB |
| Hall, William W. Browse this author |
| Takada, Ayato Browse this author →KAKEN DB |
| Abe, Takashi Browse this author →KAKEN DB |
| Wolfinger, Michael T. Browse this author |
| Simuunza, Martin Browse this author |
| Sawa, Hirofumi Browse this author →KAKEN DB |
| Issue Date: | 1-Mar-2021 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific reports |
| Volume: | 11 |
| Issue: | 1 |
| Start Page: | 4883 |
| Publisher DOI: | 10.1038/s41598-021-84365-9 |
| Abstract: | Tick-borne flaviviruses (TBFVs) infect mammalian hosts through tick bites and can cause various serious illnesses, such as encephalitis and hemorrhagic fevers, both in humans and animals. Despite their importance to public health, there is limited epidemiological information on TBFV infection in Africa. Herein, we report that a novel flavivirus, Mpulungu flavivirus (MPFV), was discovered in a Rhipicephalus muhsamae tick in Zambia. MPFV was found to be genetically related to Ngoye virus detected in ticks in Senegal, and these viruses formed a unique lineage in the genus Flavivirus. Analyses of dinucleotide contents of flaviviruses indicated that MPFV was similar to those of other TBFVs with a typical vertebrate genome signature, suggesting that MPFV may infect vertebrate hosts. Bioinformatic analyses of the secondary structures in the 3 ' -untranslated regions (UTRs) revealed that MPFV exhibited unique exoribonuclease-resistant RNA (xrRNA) structures. Utilizing biochemical approaches, we clarified that two xrRNA structures of MPFV in the 3 ' -UTR could prevent exoribonuclease activity. In summary, our findings provide new information regarding the geographical distribution of TBFV and xrRNA structures in the 3 ' -UTR of flaviviruses. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81213 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
