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Three-dimensional visualization of mitochondria in conidia of Pyricularia oryzae using green fluorescent protein (GFP) fused with citrate synthase (CitA)

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/81224

Title: Three-dimensional visualization of mitochondria in conidia of Pyricularia oryzae using green fluorescent protein (GFP) fused with citrate synthase (CitA)
Authors: Syib'li, Muhammad Akhid Browse this author
Kodama, Aoi Browse this author
Abe, Ayumi Browse this author →KAKEN DB
Sone, Teruo Browse this author →KAKEN DB
Keywords: Pyricularia oryzae
Mitochondria
Citrate synthase (CitA)-GFP
3D visualization
Issue Date: 12-May-2020
Publisher: Springer
Journal Title: Journal of general plant pathology
Publisher DOI: 10.1007/s10327-020-00928-0
Abstract: The mitochondrion is the target organelle for QoI (quinone outside inhibitor) antifungal drugs, but mitochondrial behavior in Pyricularia oryzae remains unclear. Conidial mitochondria in fungal isolate transformed with citrate synthase (CitA)-GFP fusion gene, were observed in situ using laser-scanning confocal microscopy and computer analysis. After mycelium was harvested at 36 h and aerial mycelia removed, conidia with 1, 2, or 3 cells were observed over time. The 3D visualization of mitochondria in these conidia showed that mitochondria were initially shaped like dots and developed into a network as the conidia aged.
Rights: This is a post-peer-review, pre-copyedit version of an article published in Journal of general plant pathology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10327-020-00928-0
Type: article (author version)
URI: http://hdl.handle.net/2115/81224
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 曾根 輝雄

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