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Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells

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Title: Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells
Authors: Yeap, Swee Keong Browse this author
Mohd Ali, Norlaily Browse this author
Akhtar, Muhammad Nadeem Browse this author
Razak, Nursyamirah Abd Browse this author
Chong, Zhi Xiong Browse this author
Ho, Wan Yong Browse this author
Boo, Lily Browse this author
Zareen, Seema Browse this author
Kurniawan, Tonni Agustiono Browse this author
Avtar, Ram Browse this author →KAKEN DB
Ng, Stephanie Y. L. Browse this author
Ong, Alan Han Kiat Browse this author
Alitheen, Noorjahan Banu Browse this author
Keywords: breast cancer
2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC)
Issue Date: 1-Mar-2021
Publisher: MDPI
Journal Title: Molecules
Volume: 26
Issue: 5
Start Page: 1277
Publisher DOI: 10.3390/molecules26051277
Abstract: (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes.
Type: article
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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