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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
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Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells
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| Title: | Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells |
| Authors: | Yeap, Swee Keong Browse this author | | Mohd Ali, Norlaily Browse this author | | Akhtar, Muhammad Nadeem Browse this author | | Razak, Nursyamirah Abd Browse this author | | Chong, Zhi Xiong Browse this author | | Ho, Wan Yong Browse this author | | Boo, Lily Browse this author | | Zareen, Seema Browse this author | | Kurniawan, Tonni Agustiono Browse this author | | Avtar, Ram Browse this author →KAKEN DB | | Ng, Stephanie Y. L. Browse this author | | Ong, Alan Han Kiat Browse this author | | Alitheen, Noorjahan Banu Browse this author |
| Keywords: | breast cancer | | 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) | | MCF-7 | | apoptosis | | miRNA |
| Issue Date: | 1-Mar-2021 |
| Publisher: | MDPI |
| Journal Title: | Molecules |
| Volume: | 26 |
| Issue: | 5 |
| Start Page: | 1277 |
| Publisher DOI: | 10.3390/molecules26051277 |
| Abstract: | (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81239 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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