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Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis

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Title: Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis
Authors: Guo, Zhihui Browse this author
Xu, Meilan Browse this author
Nagano, Hironori Browse this author
Clark, Lindsay V. Browse this author
Sacks, Erik J. Browse this author
Yamada, Toshihiko Browse this author
Keywords: Miscanthus sinensis
gene expression
flowering time
sequence diversity
geographic distribution
Issue Date: 19-Feb-2021
Publisher: MDPI
Journal Title: Genes
Volume: 12
Issue: 2
Start Page: 288
Publisher DOI: 10.3390/genes12020288
Abstract: The optimal flowering time for bioenergy crop Miscanthus is essential for environmental adaptability and biomass accumulation. However, little is known about how genes controlling flowering in other grasses contribute to flowering regulation in Miscanthus. Here, we report on the sequence characterization and gene expression of Miscanthus sinensis Ghd8, a transcription factor encoding a HAP3/NF-YB DNA-binding domain, which has been identified as a major quantitative trait locus in rice, with pleiotropic effects on grain yield, heading date and plant height. In M. sinensis, we identified two homoeologous loci, MsiGhd8A located on chromosome 13 and MsiGhd8B on chromosome 7, with one on each of this paleo-allotetraploid species' subgenomes. A total of 46 alleles and 28 predicted protein sequence types were identified in 12 wild-collected accessions. Several variants of MsiGhd8 showed a geographic and latitudinal distribution. Quantitative real-time PCR revealed that MsiGhd8 expressed under both long days and short days, and MsiGhd8B showed a significantly higher expression than MsiGhd8A. The comparison between flowering time and gene expression indicated that MsiGhd8B affected flowering time in response to day length for some accessions. This study provides insight into the conserved function of Ghd8 in the Poaceae, and is an important initial step in elucidating the flowering regulatory network of Miscanthus.
Type: article
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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