|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >
Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis |
| Authors: | Guo, Zhihui Browse this author | | Xu, Meilan Browse this author | | Nagano, Hironori Browse this author | | Clark, Lindsay V. Browse this author | | Sacks, Erik J. Browse this author | | Yamada, Toshihiko Browse this author |
| Keywords: | Miscanthus sinensis | | Ghd8 | | gene expression | | flowering time | | sequence diversity | | geographic distribution |
| Issue Date: | 19-Feb-2021 |
| Publisher: | MDPI |
| Journal Title: | Genes |
| Volume: | 12 |
| Issue: | 2 |
| Start Page: | 288 |
| Publisher DOI: | 10.3390/genes12020288 |
| Abstract: | The optimal flowering time for bioenergy crop Miscanthus is essential for environmental adaptability and biomass accumulation. However, little is known about how genes controlling flowering in other grasses contribute to flowering regulation in Miscanthus. Here, we report on the sequence characterization and gene expression of Miscanthus sinensis Ghd8, a transcription factor encoding a HAP3/NF-YB DNA-binding domain, which has been identified as a major quantitative trait locus in rice, with pleiotropic effects on grain yield, heading date and plant height. In M. sinensis, we identified two homoeologous loci, MsiGhd8A located on chromosome 13 and MsiGhd8B on chromosome 7, with one on each of this paleo-allotetraploid species' subgenomes. A total of 46 alleles and 28 predicted protein sequence types were identified in 12 wild-collected accessions. Several variants of MsiGhd8 showed a geographic and latitudinal distribution. Quantitative real-time PCR revealed that MsiGhd8 expressed under both long days and short days, and MsiGhd8B showed a significantly higher expression than MsiGhd8A. The comparison between flowering time and gene expression indicated that MsiGhd8B affected flowering time in response to day length for some accessions. This study provides insight into the conserved function of Ghd8 in the Poaceae, and is an important initial step in elucidating the flowering regulatory network of Miscanthus. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81356 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
