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Evidence for exposure of asymptomatic domestic pigs to African swine fever virus during an inter-epidemic period in Zambia

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Title: Evidence for exposure of asymptomatic domestic pigs to African swine fever virus during an inter-epidemic period in Zambia
Authors: Chambaro, Herman M. Browse this author
Sasaki, Michihito Browse this author →KAKEN DB
Sinkala, Yona Browse this author
Gonzalez, Gabriel Browse this author
Squarre, David Browse this author
Fandamu, Paul Browse this author
Lubaba, Caesar Browse this author
Mataa, Liywalii Browse this author
Shawa, Misheck Browse this author
Mwape, Kabemba E. Browse this author
Gabriel, Sarah Browse this author
Chembensofu, Mwelwa Browse this author
Carr, Michael J. Browse this author →KAKEN DB
Hall, William W. Browse this author
Qiu, Yongjin Browse this author
Kajihara, Masahiro Browse this author →KAKEN DB
Takada, Ayato Browse this author →KAKEN DB
Orba, Yasuko Browse this author →KAKEN DB
Simulundu, Edgar Browse this author
Sawa, Hirofumi Browse this author →KAKEN DB
Keywords: African swine fever
Ornithodoros moubata
Issue Date: 20-May-2020
Publisher: John Wiley & Sons
Journal Title: Transboundary and emerging diseases
Volume: 67
Issue: 6
Start Page: 2741
End Page: 2752
Publisher DOI: 10.1111/tbed.13630
Abstract: African swine fever (ASF) causes persistent outbreaks in endemic and non-endemic regions in Zambia. However, the epidemiology of the disease is poorly understood, particularly during the inter-epidemic periods. We conducted surveillance for ASF in asymptomatic domestic pigs and soft ticks in selected Zambian provinces. While serum samples (n = 1,134) were collected from crossbred pigs from all study sites between 2014 and 2017, whole blood (n = 300) was collected from both crossbred and indigenous pigs in Eastern Province (EP) in 2017. Soft ticks were collected from Mosi-oa-Tunya National Park in Southern Province (SP) in 2019. Sera were screened for antibodies against ASF by ELISA while genome detection in whole blood and soft ticks was conducted by PCR. Ticks were identified morphologically and by phylogenetic analysis of the 16S rRNA gene. Seroprevalence was highest in EP (50.9%, 95% CI [47.0-54.9]) compared to significantly lower rates in SP (2.9%, 95% CI [1.6-5.1]). No antibodies to ASFV were detected in Lusaka Province. In EP, the prevalence of ASFV genome was 11.7% (35/300), significantly higher (OR = 6.2, 95% CI [2.4-16.6]) in indigenous pigs compared to crossbred pigs. The pooled prevalence of ASFV genome in ticks was 11.0%, 95% CI [8.5-13.9]. Free-range husbandry system was the only factor that was significantly associated with seropositive (p < .0001, OR = 39.3) and PCR-positive results (p < .001, OR = 5.7). Phylogenetically, based on the p72 gene, ASFV from Ornithodoros moubata ticks detected in this study belonged to genotype I, but they separated into two distinct clusters. Besides confirming ASF endemicity in EP and the presence of ASFV-infected ticks in SP, these results provide evidence for exposure of domestic pigs to ASFV in non-endemic regions during the inter-epidemic period.
Rights: This is the peer reviewed version of the following article: Chambaro HM, Sasaki M, Sinkala Y, et al. Evidence for exposure of asymptomatic domestic pigs to African swine fever virus during an inter-epidemic period in Zambia. Transbound Emerg Dis. 2020;67:2741–2752., which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤 洋文

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