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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels
| Title: | Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels |
| Authors: | Mohamed, Wessam Mohamed Ahmed Browse this author | | Ali, Alsagher O. Browse this author | | Mahmoud, Hassan Y. A. H. Browse this author | | Omar, Mosaab A. Browse this author | | Chatanga, Elisha Browse this author | | Salim, Bashir Browse this author | | Naguib, Doaa Browse this author | | Anders, Jason L. Browse this author | | Nonaka, Nariaki Browse this author →KAKEN DB | | Moustafa, Mohamed Abdallah Mohamed Browse this author | | Nakao, Ryo Browse this author →KAKEN DB |
| Keywords: | Candidatus Anaplasma camelii | | eukaryotes | | microbiome | | Theileria | | Trypanosoma evansi |
| Issue Date: | 16-Mar-2021 |
| Publisher: | MDPI |
| Journal Title: | Pathogens |
| Volume: | 10 |
| Issue: | 3 |
| Start Page: | 351 |
| Publisher DOI: | 10.3390/pathogens10030351 |
| Abstract: | Dromedary camels (Camelus dromedarius) are widely distributed in Africa, the Middle East and northern India. In this study, we aimed to detect tick-borne pathogens through investigating prokaryotic and eukaryotic microorganisms in camel blood based on a metagenomic approach and then to characterize potentially pathogenic organisms using traditional molecular techniques. We showed that the bacteria circulating in the blood of camels is dominated by Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. At the genus level, Sediminibacterium, Hydrotalea, Bradyrhizobium and Anaplasma were the most abundant taxa. Eukaryotic profile was dominated by Fungi, Charophyta and Apicomplexa. At the genus level, Theileria was detected in 10 out of 18 samples, while Sarcocystis, Hoplorhynchus and Stylocephalus were detected in one sample each. Our metagenomic approach was successful in the detection of several pathogens or potential pathogens including Anaplasma sp., Theileria ovis, Th. separata, Th. annulate, Th. mutans-like and uncharacterized Theileria sp. For further characterization, we provided the partial sequences of citrate synthase (gltA) and heat-shock protein (groEL) genes of Candidatus Anaplasma camelii. We also detected Trypanosoma evansi type A using polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) region. This combined metagenomic and traditional approach will contribute to a better understanding of the epidemiology of pathogens including tick-borne bacteria and protozoa in animals. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81491 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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