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Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels

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Title: Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels
Authors: Mohamed, Wessam Mohamed Ahmed Browse this author
Ali, Alsagher O. Browse this author
Mahmoud, Hassan Y. A. H. Browse this author
Omar, Mosaab A. Browse this author
Chatanga, Elisha Browse this author
Salim, Bashir Browse this author
Naguib, Doaa Browse this author
Anders, Jason L. Browse this author
Nonaka, Nariaki Browse this author →KAKEN DB
Moustafa, Mohamed Abdallah Mohamed Browse this author
Nakao, Ryo Browse this author →KAKEN DB
Keywords: Candidatus Anaplasma camelii
Trypanosoma evansi
Issue Date: 16-Mar-2021
Publisher: MDPI
Journal Title: Pathogens
Volume: 10
Issue: 3
Start Page: 351
Publisher DOI: 10.3390/pathogens10030351
Abstract: Dromedary camels (Camelus dromedarius) are widely distributed in Africa, the Middle East and northern India. In this study, we aimed to detect tick-borne pathogens through investigating prokaryotic and eukaryotic microorganisms in camel blood based on a metagenomic approach and then to characterize potentially pathogenic organisms using traditional molecular techniques. We showed that the bacteria circulating in the blood of camels is dominated by Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. At the genus level, Sediminibacterium, Hydrotalea, Bradyrhizobium and Anaplasma were the most abundant taxa. Eukaryotic profile was dominated by Fungi, Charophyta and Apicomplexa. At the genus level, Theileria was detected in 10 out of 18 samples, while Sarcocystis, Hoplorhynchus and Stylocephalus were detected in one sample each. Our metagenomic approach was successful in the detection of several pathogens or potential pathogens including Anaplasma sp., Theileria ovis, Th. separata, Th. annulate, Th. mutans-like and uncharacterized Theileria sp. For further characterization, we provided the partial sequences of citrate synthase (gltA) and heat-shock protein (groEL) genes of Candidatus Anaplasma camelii. We also detected Trypanosoma evansi type A using polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) region. This combined metagenomic and traditional approach will contribute to a better understanding of the epidemiology of pathogens including tick-borne bacteria and protozoa in animals.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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