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TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein

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Title: TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein
Authors: Kishimoto, Mai Browse this author
Uemura, Kentaro Browse this author
Sanaki, Takao Browse this author
Sato, Akihiko Browse this author
Hall, William W. Browse this author
Kariwa, Hiroaki Browse this author →KAKEN DB
Orba, Yasuko Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Sasaki, Michihito Browse this author →KAKEN DB
Keywords: severe acute respiratory syndrome-like coronavirus-2 (SARS-CoV-2)
type II transmembrane serine protease (TTSP)
spike protein
Issue Date: 28-Feb-2021
Publisher: MDPI
Journal Title: Viruses-Basel
Volume: 13
Issue: 3
Start Page: 384
Publisher DOI: 10.3390/v13030384
Abstract: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) utilizes host proteases, including a plasma membrane-associated transmembrane protease, serine 2 (TMPRSS2) to cleave and activate the virus spike protein to facilitate cellular entry. Although TMPRSS2 is a well-characterized type II transmembrane serine protease (TTSP), the role of other TTSPs on the replication of SARS-CoV-2 remains to be elucidated. Here, we have screened 12 TTSPs using human angiotensin-converting enzyme 2-expressing HEK293T (293T-ACE2) cells and Vero E6 cells and demonstrated that exogenous expression of TMPRSS11D and TMPRSS13 enhanced cellular uptake and subsequent replication of SARS-CoV-2. In addition, SARS-CoV-1 and SARS-CoV-2 share the same TTSPs in the viral entry process. Our study demonstrates the impact of host TTSPs on infection of SARS-CoV-2, which may have implications for cell and tissue tropism, for pathogenicity, and potentially for vaccine development.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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