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Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens
| Title: | Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens |
| Authors: | Yamaguchi, Kazushi Browse this author | | Otomo, Kohei Browse this author →KAKEN DB | | Kozawa, Yuichi Browse this author →KAKEN DB | | Tsutsumi, Motosuke Browse this author →KAKEN DB | | Inose, Tomoko Browse this author →KAKEN DB | | Hirai, Kenji Browse this author →KAKEN DB | | Sato, Shunichi Browse this author →KAKEN DB | | Nemoto, Tomomi Browse this author →KAKEN DB | | Uji-I, Hiroshi Browse this author →KAKEN DB |
| Issue Date: | 12-Jan-2021 |
| Publisher: | American Chemical Society |
| Journal Title: | ACS Omega |
| Volume: | 6 |
| Issue: | 1 |
| Start Page: | 438 |
| End Page: | 447 |
| Publisher DOI: | 10.1021/acsomega.0c04888 |
| Abstract: | We developed adaptive optical (AO) two-photon excitation microscopy by introducing a spatial light modulator (SLM) in a commercially available microscopy system. For correcting optical aberrations caused by refractive index (RI) interfaces at a specimen's surface, spatial phase distributions of the incident excitation laser light were calculated using 3D coordination of the RI interface with a 3D ray-tracing method. Based on the calculation, we applied a 2D phase-shift distribution to a SLM and achieved the proper point spread function. AO two-photon microscopy improved the fluorescence image contrast in optical phantom mimicking biological specimens. Furthermore, it enhanced the fluorescence intensity from tubulin-labeling dyes in living multicellular tumor spheroids and allowed successful visualization of dendritic spines in the cortical layer V of living mouse brains in the secondary motor region with a curved surface. The AO approach is useful for observing dynamic physiological activities in deep regions of various living biological specimens with curved surfaces. |
| Rights: | Copyright © 2020 The Authors. Published by American Chemical Society |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81527 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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