HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Research Institute for Electronic Science >
Peer-reviewed Journal Articles, etc >

Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens

Files in This Item:

The file(s) associated with this item can be obtained from the following URL:

Title: Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens
Authors: Yamaguchi, Kazushi Browse this author
Otomo, Kohei Browse this author →KAKEN DB
Kozawa, Yuichi Browse this author →KAKEN DB
Tsutsumi, Motosuke Browse this author →KAKEN DB
Inose, Tomoko Browse this author →KAKEN DB
Hirai, Kenji Browse this author →KAKEN DB
Sato, Shunichi Browse this author →KAKEN DB
Nemoto, Tomomi Browse this author →KAKEN DB
Uji-I, Hiroshi Browse this author →KAKEN DB
Issue Date: 12-Jan-2021
Publisher: American Chemical Society
Journal Title: ACS Omega
Volume: 6
Issue: 1
Start Page: 438
End Page: 447
Publisher DOI: 10.1021/acsomega.0c04888
Abstract: We developed adaptive optical (AO) two-photon excitation microscopy by introducing a spatial light modulator (SLM) in a commercially available microscopy system. For correcting optical aberrations caused by refractive index (RI) interfaces at a specimen's surface, spatial phase distributions of the incident excitation laser light were calculated using 3D coordination of the RI interface with a 3D ray-tracing method. Based on the calculation, we applied a 2D phase-shift distribution to a SLM and achieved the proper point spread function. AO two-photon microscopy improved the fluorescence image contrast in optical phantom mimicking biological specimens. Furthermore, it enhanced the fluorescence intensity from tubulin-labeling dyes in living multicellular tumor spheroids and allowed successful visualization of dendritic spines in the cortical layer V of living mouse brains in the secondary motor region with a curved surface. The AO approach is useful for observing dynamic physiological activities in deep regions of various living biological specimens with curved surfaces.
Rights: Copyright © 2020 The Authors. Published by American Chemical Society
Type: article
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University