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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
Photodynamic inactivation of oral bacteria with silver nanoclusters/rose bengal nanocomposite
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| Title: | Photodynamic inactivation of oral bacteria with silver nanoclusters/rose bengal nanocomposite |
| Authors: | Shitomi, Kanako Browse this author →KAKEN DB | | Miyaji, Hirofumi Browse this author →KAKEN DB | | Miyata, Saori Browse this author | | Sugaya, Tsutomu Browse this author →KAKEN DB | | Ushijima, Natsumi Browse this author | | Akasaka, Tsukasa Browse this author →KAKEN DB | | Kawasaki, Hideya Browse this author →KAKEN DB |
| Keywords: | Aggregatibacter actinomycetemcomitans | | Silver nanoclusters (AgNCs)/rose bengal (RB) nanocomposite | | Antibacterial photodynamic therapy | | Porphyromonas gingivalis | | Singlet oxygen | | Streptococcus mutans |
| Issue Date: | Jun-2020 |
| Publisher: | Elsevier |
| Journal Title: | Photodiagnosis and Photodynamic Therapy |
| Volume: | 30 |
| Start Page: | 101647 |
| Publisher DOI: | 10.1016/j.pdpdt.2019.101647 |
| PMID: | 31904554 |
| Abstract: | Antimicrobial photodynamic therapy (a-PDT) is a promising anti-infective technique for generation of singlet oxygen (1O2) to target dental disease. However, conventional organic photosensitizers have problems for clinical use in terms of cytotoxicity, quenching of a-PDT activity by self-dimerization, and the lack of long-term antibacterial effect. We herein propose silver nanoclusters/rose bengal nanocomposite (AgNCs/RB) as a novel photosensitizer with two primary antibacterial effects: (1) 1O2 generation by irradiated RB and (2) Ag+ ion release from AgNCs. AgNCs/RB irradiated with white light-emitting diode (LED) for a short irradiation time of 1 min significantly decreased the bacterial turbidity of Streptococcus mutans, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans (P < 0.05). In SEM, TEM and LIVE/DEAD staining images, photoexcited AgNCs/RB reduced S. mutans colonization, destroyed the cell membrane, and increased the number of dead cells. The antibacterial efficiency of photoexcited AgNCs/RB was greater than that of AgNCs or RB alone (P < 0.05), suggesting a synergistic effect of 1O2 and Ag+ ions from photoexcited AgNCs/RB. By contrast, photoexcited AgNCs/RB did not affect WST-8 and LDH activities and morphology of NIH3T3 mammalian cells, indicating low cytotoxicity. Interestingly, the antibacterial activity of AgNCs/RB on S. mutans was maintained even after the cessation of LED irradiation, indicating a long-term antibacterial effect due to released Ag+ ions. The present AgNCs/RB photosensitizers provide effective synergistic antibacterial effects for dental a-PDT via 1O2 and Ag+ ions coupled with low cytotoxicity. |
| Rights: | ©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/81582 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮治 裕史
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