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Photodynamic inactivation of oral bacteria with silver nanoclusters/rose bengal nanocomposite

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Title: Photodynamic inactivation of oral bacteria with silver nanoclusters/rose bengal nanocomposite
Authors: Shitomi, Kanako Browse this author →KAKEN DB
Miyaji, Hirofumi Browse this author →KAKEN DB
Miyata, Saori Browse this author
Sugaya, Tsutomu Browse this author →KAKEN DB
Ushijima, Natsumi Browse this author
Akasaka, Tsukasa Browse this author →KAKEN DB
Kawasaki, Hideya Browse this author →KAKEN DB
Keywords: Aggregatibacter actinomycetemcomitans
Silver nanoclusters (AgNCs)/rose bengal (RB) nanocomposite
Antibacterial photodynamic therapy
Porphyromonas gingivalis
Singlet oxygen
Streptococcus mutans
Issue Date: Jun-2020
Publisher: Elsevier
Journal Title: Photodiagnosis and Photodynamic Therapy
Volume: 30
Start Page: 101647
Publisher DOI: 10.1016/j.pdpdt.2019.101647
PMID: 31904554
Abstract: Antimicrobial photodynamic therapy (a-PDT) is a promising anti-infective technique for generation of singlet oxygen (1O2) to target dental disease. However, conventional organic photosensitizers have problems for clinical use in terms of cytotoxicity, quenching of a-PDT activity by self-dimerization, and the lack of long-term antibacterial effect. We herein propose silver nanoclusters/rose bengal nanocomposite (AgNCs/RB) as a novel photosensitizer with two primary antibacterial effects: (1) 1O2 generation by irradiated RB and (2) Ag+ ion release from AgNCs. AgNCs/RB irradiated with white light-emitting diode (LED) for a short irradiation time of 1 min significantly decreased the bacterial turbidity of Streptococcus mutans, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans (P < 0.05). In SEM, TEM and LIVE/DEAD staining images, photoexcited AgNCs/RB reduced S. mutans colonization, destroyed the cell membrane, and increased the number of dead cells. The antibacterial efficiency of photoexcited AgNCs/RB was greater than that of AgNCs or RB alone (P < 0.05), suggesting a synergistic effect of 1O2 and Ag+ ions from photoexcited AgNCs/RB. By contrast, photoexcited AgNCs/RB did not affect WST-8 and LDH activities and morphology of NIH3T3 mammalian cells, indicating low cytotoxicity. Interestingly, the antibacterial activity of AgNCs/RB on S. mutans was maintained even after the cessation of LED irradiation, indicating a long-term antibacterial effect due to released Ag+ ions. The present AgNCs/RB photosensitizers provide effective synergistic antibacterial effects for dental a-PDT via 1O2 and Ag+ ions coupled with low cytotoxicity.
Rights: ©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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