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In Silico Analysis of ACE Inhibitory Peptides from Chloroplast Proteins of Red Alga Grateloupia asiatica

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Title: In Silico Analysis of ACE Inhibitory Peptides from Chloroplast Proteins of Red Alga Grateloupia asiatica
Authors: Sumikawa, Kana Browse this author
Takei, Kentaro Browse this author
Kumagai, Yuya Browse this author →KAKEN DB
Shimizu, Takeshi Browse this author
Yasui, Hajime Browse this author →KAKEN DB
Kishimura, Hideki Browse this author →KAKEN DB
Keywords: Red alga
Grateloupia asiatica
ACE inhibitory peptide
Chloroplast genome
In silico analysis
Issue Date: Jun-2020
Publisher: Springer
Journal Title: Marine Biotechnology
Volume: 22
Issue: 3
Start Page: 391
End Page: 402
Publisher DOI: 10.1007/s10126-020-09959-2
Abstract: Inhibition of angiotensin I-converting enzyme (ACE) is one of the key factors to repress high blood pressure. Although many studies have been reported that seaweed protein hydrolysates showed the ACE inhibitory activity, the comprehensive understanding of the relationship was still unclear. In this study, we employed chloroplast genome for in silico analysis and compared it with in vitro experiments. We first extracted water-soluble proteins (WSP) from red alga Grateloupia asiatica, which contained mainly PE, PC, APC, and Rbc, and prepared WSP hydrolysate by thermolysin, resulting that the hydrolysate showed ACE inhibitory activity. Then, we determined the complete chloroplast genome of G. asiatica (187,518 bp: 206 protein-coding genes, 29 tRNA, and 3 rRNA) and clarified the amino acid sequences of main WSP, i.e., phycobiliproteins and Rubisco, to perform in silico analysis. Consequently, 190 potential ACE inhibitory peptides existed in the main WSP sequences, and 21 peptides were obtained by in silico thermolysin digestion. By comparing in vitro and in silico analyses, in vitro ACE inhibitory activity was correlated to the IC50 value from in silico digestion. Therefore, in silico approach provides insight into the comprehensive understanding of the potential bioactive peptides from seaweed proteins.
Rights: This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at:
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岸村 栄毅

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