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Diverse mosquito-specific flaviviruses in the Bolivian Amazon basin

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Title: Diverse mosquito-specific flaviviruses in the Bolivian Amazon basin
Authors: Orba, Yasuko Browse this author →KAKEN DB
Matsuno, Keita Browse this author →KAKEN DB
Nakao, Ryo Browse this author
Kryukov, Kirill Browse this author
Saito, Yumi Browse this author
Kawamori, Fumihiko Browse this author
Vega, Ariel Loza Browse this author
Watanabe, Tokiko Browse this author
Maemura, Tadashi Browse this author
Sasaki, Michihito Browse this author
Hall, William W. Browse this author
Hall, Roy A. Browse this author
Pereira, Juan Antonio Browse this author
Nakagawa, So Browse this author
Sawa, Hirofumi Browse this author →KAKEN DB
Keywords: Amazon
insect-specific flavivirus
Issue Date: 8-Jan-2021
Publisher: Society for General Microbiology
Journal Title: Journal of general virology
Volume: 102
Issue: 3
Start Page: 001518
Publisher DOI: 10.1099/jgv.0.001518
Abstract: The genus Flavivirus includes a range of mosquito-specific viruses in addition to well-known medically important arboviruses. Isolation and comprehensive genomic analyses of viruses in mosquitoes collected in Bolivia resulted in the identification of three novel flavivirus species. Psorophora flavivirus (PSFV) was isolated from Psorophora albigenu. The coding sequence of the PSFV polyprotein shares 60% identity with that of the Aedes-associated lineage II insect-specific flavivirus (ISF), Marisma virus. Isolated PSFV replicates in both Aedes albopictus- and Aedes aegypti-derived cells, but not in mammalian Vero or BHK-21 cell lines. Two other flaviviruses, Ochlerotatus scapularis flavivirus (OSFV) and Mansonia flavivirus (MAFV), which were identified from Ochlerotatus scapularis and Mansonia titillans, respectively, group with the classical lineage I ISFs. The protein coding sequences of these viruses share only 60 and 40% identity with the most closely related of known lineage I ISFs, including Xishuangbanna aedes flavivirus and Sabethes flavivirus, respectively. Phylogenetic analysis suggests that MAFV is clearly distinct from the groups of the current known Culicinae-associated lineage I ISFs. Interestingly, the predicted amino acid sequence of the MAFV capsid protein is approximately two times longer than that of any of the other known flaviviruses. Our results indicate that flaviviruses with distinct features can be found at the edge of the Bolivian Amazon basin at sites that are also home to dense populations of human-biting mosquitoes.
Rights: © Orba, Yasuko; Matsuno, Keita; Nakao, Ryo; Kryukov, Kirill; Saito, Yumi; Kawamori, Fumihiko; Vega, Ariel Loza; Watanabe, Tokiko; Maemura, Tadashi; Sasaki, Michihito; Hall, William W.; Hall, Roy A.; Pereira, Juan Antonio; Nakagawa, So; Sawa, Hirofumi, 2021. The definitive peer reviewed, edited version of this article is published in Journal of general virology, 102 (3), 2021,
Type: article (author version)
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大場 靖子

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