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Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle

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Title: Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle
Authors: Torii, Shiho Browse this author
Orba, Yasuko Browse this author →KAKEN DB
Sasaki, Michihito Browse this author →KAKEN DB
Tabata, Koshiro Browse this author
Wada, Yuji Browse this author
Carr, Michael Browse this author →KAKEN DB
Hobson-Peters, Jody Browse this author
Hall, Roy A. Browse this author
Takada, Ayato Browse this author →KAKEN DB
Fukuhara, Takasuke Browse this author →KAKEN DB
Matsuura, Yoshiharu Browse this author →KAKEN DB
Hall, William W. Browse this author
Sawa, Hirofumi Browse this author →KAKEN DB
Keywords: Chikungunya virus
endosomal sorting complexes required for transport (ESCRT)
viral replication
hepatocyte growth factor-regulated tyrosine kinase substrate (HGS)
viral release
host factor
siRNA screen
particle release
Issue Date: 5-Jun-2020
Publisher: American Society for Biochemistry and Molecular Biology (ASBMB)
Journal Title: Journal of Biological Chemistry (JBC)
Volume: 295
Issue: 23
Start Page: 7941
End Page: 7957
Publisher DOI: 10.1074/jbc.RA119.012303
Abstract: Chikungunya fever is a re-emerging zoonotic disease caused by chikungunya virus (CHIKV), a member of the Alphavirus genus in the Togaviridae family. Only a few studies have reported on the host factors required for intracellular CHIKV trafficking. Here, we conducted an imaging-based siRNA screen to identify human host factors for intracellular trafficking that are involved in CHIKV infection, examined their interactions with CHIKV proteins, and investigated the contributions of these proteins to CHIKV infection. The results of the siRNA screen revealed that host endosomal sorting complexes required for transport (ESCRT) proteins are recruited during CHIKV infection. Co-immunoprecipitation analyses revealed that both structural and nonstructural CHIKV proteins interact with hepatocyte growth factor?regulated tyrosine kinase substrate (HGS), a component of the ESCRT-0 complex. We also observed that HGS co-localizes with the E2 protein of CHIKV and with dsRNA, a marker of the replicated CHIKV genome. Results from gene knockdown analyses indicated that, along with other ESCRT factors, HGS facilitates both genome replication and post-translational steps during CHIKV infection. Moreover, we show that ESCRT factors are also required for infections with other alphaviruses. We conclude that during CHIKV infection, several ESCRT factors are recruited via HGS and are involved in viral genome replication and post-translational processing of viral proteins.
Rights: This research was originally published in Journal of Biological Chemistry. Shiho Torii, Yasuko Orba, Michihito Sasaki, Koshiro Tabata, Yuji Wada, Michael Carr, Jody Hobson-Peters, Roy A. Hall, Ayato Takada, Takasuke Fukuhara, Yoshiharu Matsuura, William W. Hall and Hirofumi Sawa. Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle. Journal of Biological Chemistry. 2020; Vol.295:7941-7957. ©the American Society for Biochemistry and Molecular Biology.
Type: article
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤 洋文

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