| Title: | Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle |
| Authors: | Torii, Shiho Browse this author |
| Orba, Yasuko Browse this author →KAKEN DB |
| Sasaki, Michihito Browse this author →KAKEN DB |
| Tabata, Koshiro Browse this author |
| Wada, Yuji Browse this author |
| Carr, Michael Browse this author →KAKEN DB |
| Hobson-Peters, Jody Browse this author |
| Hall, Roy A. Browse this author |
| Takada, Ayato Browse this author →KAKEN DB |
| Fukuhara, Takasuke Browse this author →KAKEN DB |
| Matsuura, Yoshiharu Browse this author →KAKEN DB |
| Hall, William W. Browse this author |
| Sawa, Hirofumi Browse this author →KAKEN DB |
| Keywords: | Chikungunya virus |
| endosomal sorting complexes required for transport (ESCRT) |
| alphavirus |
| trafficking |
| viral replication |
| hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) |
| viral release |
| host factor |
| siRNA screen |
| particle release |
| host |
| Issue Date: | 5-Jun-2020 |
| Publisher: | American Society for Biochemistry and Molecular Biology (ASBMB) |
| Journal Title: | Journal of Biological Chemistry (JBC) |
| Volume: | 295 |
| Issue: | 23 |
| Start Page: | 7941 |
| End Page: | 7957 |
| Publisher DOI: | 10.1074/jbc.RA119.012303 |
| Abstract: | Chikungunya fever is a re-emerging zoonotic disease caused by chikungunya virus (CHIKV), a member of the Alphavirus genus in the Togaviridae family. Only a few studies have reported on the host factors required for intracellular CHIKV trafficking. Here, we conducted an imaging-based siRNA screen to identify human host factors for intracellular trafficking that are involved in CHIKV infection, examined their interactions with CHIKV proteins, and investigated the contributions of these proteins to CHIKV infection. The results of the siRNA screen revealed that host endosomal sorting complexes required for transport (ESCRT) proteins are recruited during CHIKV infection. Co-immunoprecipitation analyses revealed that both structural and nonstructural CHIKV proteins interact with hepatocyte growth factor?regulated tyrosine kinase substrate (HGS), a component of the ESCRT-0 complex. We also observed that HGS co-localizes with the E2 protein of CHIKV and with dsRNA, a marker of the replicated CHIKV genome. Results from gene knockdown analyses indicated that, along with other ESCRT factors, HGS facilitates both genome replication and post-translational steps during CHIKV infection. Moreover, we show that ESCRT factors are also required for infections with other alphaviruses. We conclude that during CHIKV infection, several ESCRT factors are recruited via HGS and are involved in viral genome replication and post-translational processing of viral proteins. |
| Rights: | This research was originally published in Journal of Biological Chemistry. Shiho Torii, Yasuko Orba, Michihito Sasaki, Koshiro Tabata, Yuji Wada, Michael Carr, Jody Hobson-Peters, Roy A. Hall, Ayato Takada, Takasuke Fukuhara, Yoshiharu Matsuura, William W. Hall and Hirofumi Sawa. Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle. Journal of Biological Chemistry. 2020; Vol.295:7941-7957. ©the American Society for Biochemistry and Molecular Biology. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81721 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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