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Host serine proteases TMPRSS2 and TMPRSS11D mediate proteolytic activation and trypsin-independent infection in group A rotaviruses
| Title: | Host serine proteases TMPRSS2 and TMPRSS11D mediate proteolytic activation and trypsin-independent infection in group A rotaviruses |
| Authors: | Sasaki, Michihito Browse this author →KAKEN DB | | Itakura, Yukari Browse this author | | Kishimoto, Mai Browse this author | | Tabata, Koshiro Browse this author | | Uemura, Kentaro Browse this author | | Ito, Naoto Browse this author →KAKEN DB | | Sugiyama, Makoto Browse this author →KAKEN DB | | Wastika, Christida E Browse this author | | Orba, Yasuko Browse this author →KAKEN DB | | Sawa, Hirofumi Browse this author →KAKEN DB |
| Keywords: | group A rotavirus | | proteolytic activation | | type II transmembrane serine proteases | | VP4 | | viral entry |
| Issue Date: | Jun-2021 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Journal of virology |
| Volume: | 95 |
| Issue: | 11 |
| Start Page: | e00398-21 |
| Publisher DOI: | 10.1128/JVI.00398-21 |
| PMID: | 33762412 |
| Abstract: | Group A rotaviruses (RVAs) are representative enteric virus species and major causes of diarrhea in humans and animals. The RVA virion is a triple-layered particle, and the outermost layer consists of the glycoprotein VP7 and spike protein VP4. To increase the infectivity of RVA, VP4 is proteolytically cleaved into VP5* and VP8* subunits by trypsin; these subunits form a rigid spike structure on the virion surface. In this study, we investigated the growth of RVAs in cells transduced with type II transmembrane serine proteases (TTSPs), which cleave fusion proteins and promote infection by respiratory viruses, such as influenza viruses, paramyxoviruses, and coronaviruses. We identified TMPRSS2 and TMPRSS11D as host TTSPs that mediate trypsin-independent and multicycle infection by human and animal RVA strains. In vitro cleavage assays revealed that recombinant TMPRSS11D cleaved RVA VP4. We also found that TMPRSS2 and TMPRSS11D promote the infectious entry of immature RVA virions, but they could not activate nascent progeny virions in the late phase of infection. This observation differed from the TTSP-mediated activation process of paramyxoviruses, revealing the existence of virus species-specific activation processes in TTSPs. Our study provides new insights into the interaction between RVAs and host factors, and TTSP-transduced cells offer potential advantages for RVA research and development. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81748 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐々木 道仁
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