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Preparation of beta(1 -> 3)/beta(1 -> 4) xylooligosaccharides from red alga dulse by two xylanases from Streptomyces thermogriseus
Title: | Preparation of beta(1 -> 3)/beta(1 -> 4) xylooligosaccharides from red alga dulse by two xylanases from Streptomyces thermogriseus |
Authors: | Fujii, Yuki Browse this author | Kobayashi, Manami Browse this author | Miyabe, Yoshikatsu Browse this author | Kishimura, Hideki Browse this author | Hatanaka, Tadashi Browse this author | Kumagai, Yuya Browse this author |
Keywords: | Palmaria palmata in Japan | beta(1 -> 3)/beta(1 -> 4)-xylooligosaccharides production | GH10 and GH11 endoxylanase | Actinomycete |
Issue Date: | 12-May-2021 |
Publisher: | Springer |
Journal Title: | Bioresources and bioprocessing |
Volume: | 8 |
Issue: | 1 |
Start Page: | 38 |
Publisher DOI: | 10.1186/s40643-021-00390-6 |
Abstract: | Red alga dulse contains xylan with beta(1 -> 3)/beta(1 -> 4) linkages. We previously prepared xylooligosaccharides (XOSs) from dulse xylan; however, the product contained many D-xylose residues and fewer XOSs with beta(1 -> 3) linkages. To improve the efficiency of XOS production, we prepared two recombinant endoxylanases from Streptomyces thermogriseus (StXyl10 and StXyl11). Comparing the k(cat)/K-m values for dulse xylan, this value from StXyl10 was approximately two times higher than that from StXyl11. We then determined the suitable conditions for XOS production. As a result, dulse XOS was prepared by the successive hydrolysis of 10 mg/mL dulse xylan by 0.5 mu g/mL StXyl10 for 4 h at 50 degrees C and then 2.0 mu g/mL StXyl11 for 36 h at 60 degrees C. Xylan was converted into 95.8% XOS, including 59.7% XOS with a beta(1 -> 3) linkage and 0.97% D-xylose. Our study provides useful information for the production of XOSs with beta(1 -> 3) linkages. |
Type: | article |
URI: | http://hdl.handle.net/2115/81973 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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