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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions
| Title: | The Phenolic Antioxidant 3,5-dihydroxy-4-methoxybenzyl Alcohol (DHMBA) Prevents Enterocyte Cell Death under Oxygen-Dissolving Cold Conditions through Polyphyletic Antioxidant Actions |
| Authors: | Fukai, Moto Browse this author →KAKEN DB | | Nakayabu, Takuya Browse this author | | Ohtani, Shintaro Browse this author | | Shibata, Kengo Browse this author | | Shimada, Shingo Browse this author | | Sakamoto, Soudai Browse this author | | Fuda, Hirotoshi Browse this author | | Furukawa, Takayuki Browse this author | | Watanabe, Mitsugu Browse this author | | Hui, Shu-Ping Browse this author | | Chiba, Hitoshi Browse this author | | Shimamura, Tsuyoshi Browse this author | | Taketomi, Akinobu Browse this author |
| Keywords: | enterocytes | | IEC-6 | | cold preservation | | hypoxia | | oxidative stress | | antioxidant | | DHMBA | | mitochondria | | survival signal | | lipid peroxidation |
| Issue Date: | May-2021 |
| Publisher: | MDPI |
| Journal Title: | Journal of clinical medicine |
| Volume: | 10 |
| Issue: | 9 |
| Start Page: | 1972 |
| Publisher DOI: | 10.3390/jcm10091972 |
| Abstract: | Cold preservation in University of Wisconsin (UW) solution is not enough to maintain the viability of the small intestine, due to the oxidative stress. The novel phenolic antioxidant 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) has dual properties to reduce oxidative stress, radical scavenging, and antioxidant protein induction, in other cells. This study was designed to determine whether DHMBA reduces cold preservation injury of enterocytes, and to identify the effector site. Enterocytes were subjected to 48-h cold preservation under atmosphere in UW solution (+/- DHMBA), and then returned to normal culture to replicate reperfusion of the small intestine after cold preservation. At the end of cold preservation (ECP) and at 1, 3, 6, and 72 h after rewarming (R1h, R3h, R6h, and R72h), we evaluated cell function and the injury mechanism. The results showed that DHMBA protected mitochondrial function mainly during cold preservation, and suppressed cell death after rewarming, as shown by the MTT, ATP, mitochondrial membrane potential, LDH, and lipid peroxidation assays, together with enhanced survival signals (PI3K, Akt, p70S6K) and induction of antioxidant proteins (HO-1, NQO-1, TRX-1). We found that DHMBA mitigates the cold-induced injury of enterocytes by protecting the mitochondria through direct and indirect antioxidative activities. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82059 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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