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Hokkaido University Collection of Scholarly and Academic Papers >
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Glycine exerts dose-dependent biphasic effects on vascular development of zebrafish embryos
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| Title: | Glycine exerts dose-dependent biphasic effects on vascular development of zebrafish embryos |
| Authors: | Tsuji-Tamura, Kiyomi Browse this author →KAKEN DB | | Sato, Mari Browse this author →KAKEN DB | | Fujita, Misato Browse this author →KAKEN DB | | Tamura, Masato Browse this author →KAKEN DB |
| Keywords: | Glycine | | Angiogenesis | | Zebrafish | | VEGF | | NOS |
| Issue Date: | 25-Jun-2020 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and biophysical research communications |
| Volume: | 527 |
| Issue: | 2 |
| Start Page: | 539 |
| End Page: | 544 |
| Publisher DOI: | 10.1016/j.bbrc.2020.04.098 |
| PMID: | 32423801 |
| Abstract: | Glycine, a non-essential amino acid, is involved in both angiogenesis and anti-angiogenesis. We hypothesized that glycine would exert dose-dependent different effects on angiogenesis. In this study, we investigated the effects of a broad range of concentrations of glycine on vascular development using transgenic zebrafish Tg(fli1a:Myr-mCherry)(ncv1) embryos. Effects of glycine transporter (GlyT) inhibitors (sarcosine and bitopertin) and a glycine receptor (GlyR) inhibitor (strychnine) were also examined in embryos in the absence or presence of glycine. After exposure to glycine and inhibitors, intersegmental vessels (ISVs) were observed by fluorescent microscopy. Low concentrations of glycine promoted the development of ISVs, whereas high concentrations reduced it. These effects of glycine could generally be reversed by treatment with GlyT and GlyR inhibitors. Furthermore, expressions of vascular endothelial growth factor (VEGF) (an angiogenic factor) and nitric oxide synthase (NOS) (an enzyme for nitric oxide synthesis) were associated with the dose-dependent effects of glycine. Our results suggest that glycine exerts dose-dependent biphasic effects on vascular development, which rely on GlyTs and GlyRs, and correlate with the expression of VEGF and NOS genes. At low concentrations, glycine acted as an angiogenic factor. In contrast, at high concentrations, glycine induced anti-angiogenesis. This evidence provides a novel insight into glycine as a unique target in angiogenic and anti-angiogenic therapy. (C) 2020 Elsevier Inc. All rights reserved. |
| Rights: | ©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/82062 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田村（辻） 潔美
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