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Novel chromosomal insertions of ISEcp1-bla(CTX-M-15) and diverse antimicrobial resistance genes in Zambian clinical isolates of Enterobacter cloacae and Escherichia coli

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Title: Novel chromosomal insertions of ISEcp1-bla(CTX-M-15) and diverse antimicrobial resistance genes in Zambian clinical isolates of Enterobacter cloacae and Escherichia coli
Authors: Shawa, Misheck Browse this author
Furuta, Yoshikazu Browse this author
Mulenga, Gillan Browse this author
Mubanga, Maron Browse this author
Mulenga, Evans Browse this author
Zorigt, Tuvshinzaya Browse this author
Kaile, Christone Browse this author
Simbotwe, Manyando Browse this author
Paudel, Atmika Browse this author →KAKEN DB
Hang'ombe, Bernard Browse this author
Higashi, Hideaki Browse this author →KAKEN DB
Keywords: ISEcp1
Chromosomal insertion
Extended spectrum beta-lactamase
Enterobacter cloacae
Escherichia coli
Issue Date: 10-May-2021
Publisher: BioMed Central
Journal Title: Antimicrobial resistance and infection control
Volume: 10
Issue: 1
Start Page: 79
Publisher DOI: 10.1186/s13756-021-00941-8
Abstract: Background: The epidemiology of extended-spectrum beta-lactamases (ESBLs) has undergone dramatic changes, with CTX-M-type enzymes prevailing over other types. bla(CTX-M) genes, encoding CTX-M-type ESBLs, are usually found on plasmids, but chromosomal location is becoming common. Given that bla(CTX-M)-harboring strains often exhibit multidrug resistance (MDR), it is important to investigate the association between chromosomally integrated bla(CTX-M) and the presence of additional antimicrobial resistance (AMR) genes, and to identify other relevant genetic elements. Methods: A total of 46 clinical isolates of cefotaxime-resistant Enterobacteriaceae (1 Enterobacter cloacae, 9 Klebsiella pneumoniae, and 36 Escherichia coli) from Zambia were subjected to whole-genome sequencing (WGS) using MiSeq and MinION. By reconstructing nearly complete genomes, bla(CTX-M) genes were categorized as either chromosomal or plasmid-borne. Results: WGS-based genotyping identified 58 AMR genes, including four bla(CTX-M) alleles (i.e., bla(CTX-M-14), bla(CTX-M-15), bla(CTX-M-27), and bla(CTX-M-55)). Hierarchical clustering using selected phenotypic and genotypic characteristics suggested clonal dissemination of bla(CTX-M) genes. Out of 45 bla(CTX-M) gene-carrying strains, 7 harbored the gene in their chromosome. In one E. cloacae and three E. coli strains, chromosomal bla(CTX-M-15) was located on insertions longer than 10 kb. These insertions were bounded by ISEcp1 at one end, exhibited a high degree of nucleotide sequence homology with previously reported plasmids, and carried multiple AMR genes that corresponded with phenotypic AMR profiles. Conclusion: Our study revealed the co-occurrence of ISEcp1-bla(CTX-M-15) and multiple AMR genes on chromosomal insertions in E. cloacae and E. coli, suggesting that ISEcp1 may be responsible for the transposition of diverse AMR genes from plasmids to chromosomes. Stable retention of such insertions in chromosomes may facilitate the successful propagation of MDR clones among these Enterobacteriaceae species.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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