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Expression of Grainyhead-like 2 in the Process of Ductal Development of Mouse Mammary Gland

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Title: Expression of Grainyhead-like 2 in the Process of Ductal Development of Mouse Mammary Gland
Authors: Matsuoka, Shinya Browse this author
Suzuki, Hiroyoshi Browse this author
Kato, Chieko Browse this author
Kamikawa-Tokai, Mai Browse this author
Kamikawa, Akihiro Browse this author
Okamatsu-Ogura, Yuko Browse this author →KAKEN DB
Kimura, Kazuhiro Browse this author →KAKEN DB
Keywords: claudin
Grainyhead-like 2
mammary gland
postnatal development
Issue Date: Jun-2021
Publisher: SAGE Publications
Journal Title: Journal of histochemistry and cytochemistry
Volume: 69
Issue: 6
Start Page: 373
End Page: 388
Publisher DOI: 10.1369/00221554211013715
Abstract: Grainyhead-like 2 (Grhl2) is a transcription factor regulating cell adhesion genes. Grhl2 acts as an epithelial-mesenchymal transition suppressor, and it is a proto-oncogene involved in estrogen-stimulated breast cancer proliferation. However, its expression during ovarian hormone-dependent mammary ductal development remains obscure. We here examined Grhl2 expression in the mammary gland of normal and steroid-replaced ovariectomized mice. Grhl2 protein signals were detected in both the mammary luminal epithelial and myoepithelial nuclei. The ratio and density of Grhl2-positive nuclei increased after the onset of puberty and progressed with age, whereas Grhl2-negative epithelial cells were detected in mature ducts. Claudin 3, claudin 4, claudin 7, and E-cadherin gene expression in the mammary gland was upregulated, and their expression was highly correlated with Grhl2 gene expression. Furthermore, Grhl2 mRNA expression and ductal lumen width were significantly increased by the combined treatment of estrogen and progesterone compared with estrogen alone. These results suggest that Grhl2 expressed in the luminal epithelial and myoepithelial cells from the early phase of ductal development, controlling the expression of cell adhesion molecules to establish functional ducts:
Rights: 1. Matsuoka S, Suzuki H, Kato C, et al. Expression of Grainyhead-like 2 in the Process of Ductal Development of Mouse Mammary Gland. Journal of Histochemistry & Cytochemistry. 2021;69(6):373-388. doi:10.1369/00221554211013715
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 和弘

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