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Hokkaido University Collection of Scholarly and Academic Papers >
International Institute for Zoonosis Control >
Peer-reviewed Journal Articles, etc >
Prevalence and Characterization of Quinolone-Resistance Determinants in Escherichia coli Isolated from Food-Producing Animals and Animal-Derived Food in the Philippines
| Title: | Prevalence and Characterization of Quinolone-Resistance Determinants in Escherichia coli Isolated from Food-Producing Animals and Animal-Derived Food in the Philippines |
| Authors: | Belotindos, Lawrence Browse this author | | Villanueva, Marvin Browse this author | | Miguel, Joel Browse this author | | Bwalya, Precious Browse this author | | Harada, Tetsuya Browse this author →KAKEN DB | | Kawahara, Ryuji Browse this author →KAKEN DB | | Nakajima, Chie Browse this author →KAKEN DB | | Mingala, Claro Browse this author | | Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Keywords: | Escherichia coli | | quinolone | | QRDR | | PMQR | | the Philippines |
| Issue Date: | 9-Apr-2021 |
| Publisher: | MDPI |
| Journal Title: | Antibiotics-Basel |
| Volume: | 10 |
| Issue: | 4 |
| Start Page: | 413 |
| Publisher DOI: | 10.3390/antibiotics10040413 |
| Abstract: | Antimicrobial resistance to quinolones, which constitutes a threat to public health, has been increasing worldwide. In this study, we investigated the prevalence of quinolone-resistant determinants in Escherichia coli not susceptible to quinolones and isolated from food-producing animals and food derived from them, in the Philippines. A total of 791 E. coli strains were isolated in 56.4% of 601 beef, chicken, pork, egg, and milk samples, as well as environmental, cloacal, and rectal swab-collected samples from supermarkets, open markets, abattoirs, and poultry, swine, and buffalo farms. Using the disc diffusion method, it was determined that 78.6% and 55.4% of the isolates were resistant to at least one antimicrobial and multiple drugs, respectively. In 141 isolates not susceptible to quinolones, 115 (81.6%) harbored quinolone-resistant determinants and had mutations predominantly in the quinolone-resistance determining regions (QRDRs) of gyrA and parC. Plasmid-mediated, quinolone resistance (PMQR) and Qnr family (qnrA1, qnrB4, and qnrS1) genes were detected in all isolates. Forty-eight sequence types were identified in isolates harboring mutations in QRDR and/or PMQR genes by multilocus sequence typing analysis. Moreover, 26 isolates harboring mutations in QRDR and/or PMQR genes belonged mostly to phylogroup B1 and Enteroaggregative E. coli. In conclusion, a high prevalence of E. coli was found in food-producing animals and products derived from them, which could potentially spread high-risk clones harboring quinolone-resistance determinants. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82245 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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