HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Institute for Genetic Medicine >
Peer-reviewed Journal Articles, etc >

Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay

Files in This Item:

The file(s) associated with this item can be obtained from the following URL:

Title: Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay
Authors: Tosa, Noriko Browse this author
Ishida, Tomoko Browse this author
Yoshimatsu, Kumiko Browse this author →KAKEN DB
Hayashimoto, Nobuhito Browse this author
Shiokawa, Kanae Browse this author
Takakura, Akira Browse this author
Arikawa, Jiro Browse this author
Keywords: microbiological monitoring
multiplex immunochromatographic assay
small amount of serum
Issue Date: 13-May-2021
Publisher: 日本実験動物学会(JALAS/Japanese Association for Laboratory Animal Science)
Journal Title: Experimental animals
Volume: 70
Issue: 2
Start Page: 161
End Page: 168
Publisher DOI: 10.1538/expanim.20-0099
Abstract: Rapid and simple serologic tests that require only a small amount of blood without the euthanization of animals are valuable for microbial control in colonies of laboratory animals. In this study, we developed a multiplex immunochromatographic assay (ICA) for detection of antibodies to Sendai virus (also known as hemagglutinating virus of Japan), hantavirus, and sialodacryoadenitis virus, which are causative agents of major infectious diseases in rats. For this assay, an ICA strip was placed into a microtube containing 150 mu l PBS and either 0.75 mu l of rat serum or 1.5 mu l of whole blood. Binding antibodies were visualized by using anti-rat IgG antibody-conjugated colloidal gold. Under these conditions, the multiplex ICA simultaneously and specifically detected antibodies to multiple antigens. Positive serum samples for each infectious disease were used to evaluate the sensitivity and specificity of the multiplex ICA. The sensitivities of the multiplex ICA for Sendai virus, hantavirus, and sialodacryoadenitis virus were 100%, 100%, and 81%, respectively. No nonspecific reactions were observed in any of the 52 positive sera against heterologous antigens. In addition, 10 samples of uninfected sera did not show any bands except for the control line. These observations indicate high specificity of the multiplex ICA. Moreover, the multiplex ICA could be applied to diluted blood. These results indicate that the multiplex ICA is appropriate for rapid and simple serological testing of laboratory rats.
Type: article
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University