|
Hokkaido University Collection of Scholarly and Academic Papers >
Institute for Genetic Medicine >
Peer-reviewed Journal Articles, etc >
Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay
| Title: | Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay |
| Authors: | Tosa, Noriko Browse this author | | Ishida, Tomoko Browse this author | | Yoshimatsu, Kumiko Browse this author →KAKEN DB | | Hayashimoto, Nobuhito Browse this author | | Shiokawa, Kanae Browse this author | | Takakura, Akira Browse this author | | Arikawa, Jiro Browse this author |
| Keywords: | microbiological monitoring | | multiplex immunochromatographic assay | | rats | | small amount of serum |
| Issue Date: | 13-May-2021 |
| Publisher: | 日本実験動物学会（JALAS／Japanese Association for Laboratory Animal Science） |
| Journal Title: | Experimental animals |
| Volume: | 70 |
| Issue: | 2 |
| Start Page: | 161 |
| End Page: | 168 |
| Publisher DOI: | 10.1538/expanim.20-0099 |
| Abstract: | Rapid and simple serologic tests that require only a small amount of blood without the euthanization of animals are valuable for microbial control in colonies of laboratory animals. In this study, we developed a multiplex immunochromatographic assay (ICA) for detection of antibodies to Sendai virus (also known as hemagglutinating virus of Japan), hantavirus, and sialodacryoadenitis virus, which are causative agents of major infectious diseases in rats. For this assay, an ICA strip was placed into a microtube containing 150 mu l PBS and either 0.75 mu l of rat serum or 1.5 mu l of whole blood. Binding antibodies were visualized by using anti-rat IgG antibody-conjugated colloidal gold. Under these conditions, the multiplex ICA simultaneously and specifically detected antibodies to multiple antigens. Positive serum samples for each infectious disease were used to evaluate the sensitivity and specificity of the multiplex ICA. The sensitivities of the multiplex ICA for Sendai virus, hantavirus, and sialodacryoadenitis virus were 100%, 100%, and 81%, respectively. No nonspecific reactions were observed in any of the 52 positive sera against heterologous antigens. In addition, 10 samples of uninfected sera did not show any bands except for the control line. These observations indicate high specificity of the multiplex ICA. Moreover, the multiplex ICA could be applied to diluted blood. These results indicate that the multiplex ICA is appropriate for rapid and simple serological testing of laboratory rats. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82246 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
