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Osmotic gradients induce stable dome morphogenesis on extracellular matrix

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Title: Osmotic gradients induce stable dome morphogenesis on extracellular matrix
Authors: Ishida-Ishihara, Sumire Browse this author
Akiyama, Masakazu Browse this author
Furusawa, Kazuya Browse this author
Naguro, Isao Browse this author
Ryuno, Hiroki Browse this author
Sushida, Takamichi Browse this author
Ishihara, Seiichiro Browse this author
Haga, Hisashi Browse this author →KAKEN DB
Keywords: Osmotic gradient
Epithelial cells
Numerical simulation
Issue Date: Jul-2020
Publisher: Company of Biologists
Journal Title: Journal of Cell Science
Volume: 133
Issue: 14
Start Page: jcs243865
Publisher DOI: 10.1242/jcs.243865
Abstract: One of the fundamental processes in morphogenesis is dome formation, but many of the mechanisms involved are unexplored. Previous in vitro studies showed that an osmotic gradient is the driving factor of dome formation. However, these investigations were performed without extracellular matrix (ECM), which provides structural support to morphogenesis. With the use of ECM, we observed that basal hypertonic stress induced stable domes in vitro that have not been seen in previous studies. These domes developed as a result of ECM swelling via aquaporin water transport activity. Based on computer simulation, uneven swelling, with a positive feedback between cell stretching and enhanced water transport, was a cause of dome formation. These results indicate that osmotic gradients induce dome morphogenesis via both enhanced water transport activity and subsequent ECM swelling.
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 芳賀 永

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