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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Osmotic gradients induce stable dome morphogenesis on extracellular matrix
| Title: | Osmotic gradients induce stable dome morphogenesis on extracellular matrix |
| Authors: | Ishida-Ishihara, Sumire Browse this author | | Akiyama, Masakazu Browse this author | | Furusawa, Kazuya Browse this author | | Naguro, Isao Browse this author | | Ryuno, Hiroki Browse this author | | Sushida, Takamichi Browse this author | | Ishihara, Seiichiro Browse this author | | Haga, Hisashi Browse this author →KAKEN DB |
| Keywords: | Osmotic gradient | | Epithelial cells | | ECM | | Swelling | | Aquaporin | | Numerical simulation |
| Issue Date: | Jul-2020 |
| Publisher: | Company of Biologists |
| Journal Title: | Journal of Cell Science |
| Volume: | 133 |
| Issue: | 14 |
| Start Page: | jcs243865 |
| Publisher DOI: | 10.1242/jcs.243865 |
| Abstract: | One of the fundamental processes in morphogenesis is dome formation, but many of the mechanisms involved are unexplored. Previous in vitro studies showed that an osmotic gradient is the driving factor of dome formation. However, these investigations were performed without extracellular matrix (ECM), which provides structural support to morphogenesis. With the use of ECM, we observed that basal hypertonic stress induced stable domes in vitro that have not been seen in previous studies. These domes developed as a result of ECM swelling via aquaporin water transport activity. Based on computer simulation, uneven swelling, with a positive feedback between cell stretching and enhanced water transport, was a cause of dome formation. These results indicate that osmotic gradients induce dome morphogenesis via both enhanced water transport activity and subsequent ECM swelling. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82291 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 芳賀 永
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