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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >
Golgi-localized membrane protein AtTMN1/EMP12 functions in the deposition of rhamnogalacturonan II and I for cell growth in Arabidopsis
| Title: | Golgi-localized membrane protein AtTMN1/EMP12 functions in the deposition of rhamnogalacturonan II and I for cell growth in Arabidopsis |
| Authors: | Hiroguchi, Akihiko Browse this author | | Sakamoto, Shingo Browse this author →KAKEN DB | | Mitsuda, Nobutaka Browse this author →KAKEN DB | | Miwa, Kyoko Browse this author →KAKEN DB |
| Keywords: | Arabidopsis thallana | | boron | | cell elongation | | cellular attachment | | endomembrane protein (EMP) | | Golgi apparatus | | pectin deposition | | rhamnogalacturonan I | | rhamnogalacturonan II | | transmembrane nine protein (TMN) |
| Issue Date: | 4-May-2021 |
| Publisher: | Oxford University Press |
| Journal Title: | Journal of Experimental Botany |
| Volume: | 72 |
| Issue: | 10 |
| Start Page: | 3611 |
| End Page: | 3629 |
| Publisher DOI: | 10.1093/jxb/erab065 |
| Abstract: | Appropriate pectin deposition in cell walls is important for cell growth in plants. Rhamnogalacturonan II (RG-II) is a portion of pectic polysaccharides; its borate crosslinking is essential for maintenance of pectic networks. However, the overall process of RG-II synthesis is not fully understood. To identify a novel factor for RG-II deposition or dimerization in cell walls, we screened Arabidopsis mutants with altered boron (B)-dependent growth. The mutants exhibited alleviated disorders of primary root and stem elongation, and fertility under low B, but reduced primary root lengths under sufficient B conditions. Altered primary root elongation was associated with cell elongation changes caused by loss of function in AtTMN1 (Transmembrane Nine 1)/EMP12, which encodes a Golgi-localized membrane protein of unknown function that is conserved among eukaryotes. Mutant leaf and root dry weights were lower than those of wild-type plants, regardless of B conditions. In cell walls, AtTMN1 mutations reduced concentrations of B, RG-II specific 2-keto-3-deoxy monosaccharides, and rhamnose largely derived from rhamnogalacturonan I (RG-1), suggesting reduced RG-II and RG-1. Together, our findings demonstrate that AtTMN1 is required for the deposition of RG-II and RG-1 for cell growth and suggest that pectin modulates plant growth under low B conditions. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82312 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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