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Effects of N-acetylcysteine on root resorption after tooth replantation

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Title: Effects of N-acetylcysteine on root resorption after tooth replantation
Authors: Nishimi, Mitsuhiko Browse this author
Nakamura, Koichi Browse this author →KAKEN DB
Hisada, Akina Browse this author →KAKEN DB
Endo, Kazuki Browse this author
Ushimura, Shuya Browse this author
Yoshimura, Yoshitaka Browse this author →KAKEN DB
Yawaka, Yasutaka Browse this author →KAKEN DB
Keywords: Dental trauma
Root resorption
Issue Date: Aug-2020
Publisher: Elsevier
Journal Title: Pediatric Dental Journal
Volume: 30
Issue: 2
Start Page: 72
End Page: 79
Publisher DOI: 10.1016/j.pdj.2020.05.002
Abstract: In the case of complete luxation with dental trauma, delayed replantation leads to a higher probability of root resorption. We evaluated the efficacy of N-acetylcysteine (NAC) as a root canal medication for trauma-associated root resorption. A rat dental trauma model was used. In the test group, mesial root pulpectomy was carried out after tooth extraction. NAC was applied as a root canal medication followed by replantation. We euthanized the rats after two weeks, excised the maxilla, and prepared thin sections, followed by H-E and TRAP staining. Furthermore, RAW264.7 cells were treated with RANKL and NAC. The effects of NAC on osteoclast differentiation were investigated by determining the numbers of osteoclasts and nuclei and quantifying osteoclast-related mRNA expression levels by real-time PCR. The level of root resorption and number of odontoclasts tended to be lower in the NAC groups than in the control group. In terms of osteoclast differentiation, the total number of osteoclasts was lower in both the 5 and 10 mmol/L NAC groups than in the control group. Real-time PCR revealed that the expression levels of RANK, NFATc1, DC-STAMP, CD47, and cathepsin K were lower in the 10 mmol/L NAC group. NAC tended to inhibit root resorption and reduce the number of odontoclasts. NAC also inhibited the expression of osteoclast-related genes. Overall, NAC reduced the number of odontoclasts, inhibited bone resorption, and influenced the inhibition of osteoclast differentiation, fusion, and functions. Therefore, we suggest that NAC may be beneficial in the treatment of root resorption. (C) 2020 Japanese Society of Pediatric Dentistry. Published by Elsevier Ltd. All rights reserved.
Rights: © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中村 光一

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