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The role of RHOA signaling in trophectoderm cell-fate decision in cattle
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Title: | The role of RHOA signaling in trophectoderm cell-fate decision in cattle |
Authors: | Kohri, Nanami Browse this author | Akizawa, Hiroki Browse this author | Iisaka, Sakie Browse this author | Bai, Hanako Browse this author →KAKEN DB | Takahashi, Masashi Browse this author →KAKEN DB | Kawahara, Manabu Browse this author →KAKEN DB |
Keywords: | Trophectoderm | Blastocyst | Bovine | Hippo signaling pathway | RHOA activity | Differentiation |
Issue Date: | 6-Aug-2020 |
Publisher: | Elsevier |
Journal Title: | Biochemical and biophysical research communications |
Volume: | 528 |
Issue: | 4 |
Start Page: | 713 |
End Page: | 718 |
Publisher DOI: | 10.1016/j.bbrc.2020.05.210 |
Abstract: | Mammalian blastocysts are composed of two distinct cell lineages, namely the inner cell mass (ICM) and trophectoderm (TE). TE cells that give rise to the embryonic placenta are marked by an exclusive expression of the key determinant transcription factor, CDX2. Although Hippo signaling pathway is known to be responsible for this TE-specific expression of CDX2, the upstream regulator of this pathway in mammalian embryos is still controversial. In the present study, the involvement of the small molecular G protein, RHOA, in TE cell-fate decision in cattle was investigated. Inhibition of RHOA by the specific inhibitor, C3 transferase (C3), severely impaired the blastocyst formation. Further, C3 treatment significantly decreased the number of blastomeres with nuclearized YAP1, the prominent effector of Hippo pathway. An artificial isolation of ICM cells from blastocysts followed by the continuing culture to regenerate TE cells was conducted and showed that TE re-emergence from the isolated ICM is governed by Hippo pathway and suppressed by C3 treatment like that observed in developing embryos. Finally, the long-term exposure to C3 suggests the presence of alternative regulators of CDX2 expression other than RHOA signaling because there were still CDX2-positive cells after C3 treatment. These results demonstrated that RHOA signaling plays a significant role in TE cell-fate decision by regulating Hippo pathway in cattle. (C) 2020 Elsevier Inc. All rights reserved. |
Rights: | © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/82433 |
Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 川原 学
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