Hokkaido University Collection of Scholarly and Academic Papers >
Institute for Genetic Medicine >
Peer-reviewed Journal Articles, etc >
Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane
Title: | Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane |
Authors: | Kishimoto, Takuma Browse this author →KAKEN DB | Mioka, Tetsuo Browse this author | Itoh, Eriko Browse this author | Williams, David E. Browse this author | Andersen, Raymond J. Browse this author | Tanaka, Kazuma Browse this author →KAKEN DB |
Issue Date: | 15-Jul-2021 |
Publisher: | American Society for Cell Biology |
Journal Title: | Molecular biology of the cell |
Volume: | 32 |
Issue: | 15 |
Start Page: | 1374 |
End Page: | 1392 |
Publisher DOI: | 10.1091/mbc.E20-11-0699 |
Abstract: | Sterols are important lipid components of the plasma membrane (PM) in eukaryotic cells, but it is unknown how the PM retains sterols at a high concentration. Phospholipids are asymmetrically distributed in the PM, and phospholipid flippases play an important role in generating this phospholipid asymmetry. Here, we provide evidence that phospholipid flippases are essential for retaining ergosterol in the PM of yeast. A mutant in three flippases, Dnf1-Lem3, Dnf2-Lem3, and Dnf3-Crf1, and a membrane protein, Sfk1, showed a severe growth defect. We recently identified Sfk1 as a PM protein involved in phospholipid asymmetry. The PM of this mutant showed high permeability and low density. Staining with the sterol probe filipin and the expression of a sterol biosensor revealed that ergosterol was not retained in the PM. Instead, ergosterol accumulated in an esterified form in lipid droplets. We propose that ergosterol is retained in the PM by the asymmetrical distribution of phospholipids and the action of Sfk1. Once phospholipid asymmetry is severely disrupted, sterols might be exposed on the cytoplasmic leaflet of the PM and actively transported to the endoplasmic reticulum by sterol transfer proteins. |
Type: | article |
URI: | http://hdl.handle.net/2115/82546 |
Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|