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DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples

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Title: DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples
Authors: Izumi, Takuma Browse this author
Morioka, Yuhei Browse this author
Urayama, Syun-ichi Browse this author
Motooka, Daisuke Browse this author
Tamura, Tomokazu Browse this author
Kawagishi, Takahiro Browse this author
Kanai, Yuta Browse this author
Kobayashi, Takeshi Browse this author
Ono, Chikako Browse this author
Morinaga, Akinari Browse this author
Tomiyama, Takahiro Browse this author
Iseda, Norifumi Browse this author
Kosai, Yukiko Browse this author
Inokuchi, Shoichi Browse this author
Nakamura, Shota Browse this author
Tanaka, Tomohisa Browse this author
Moriishi, Kohji Browse this author
Kariwa, Hiroaki Browse this author
Yoshizumi, Tomoharu Browse this author
Mori, Masaki Browse this author
Matsuura, Yoshiharu Browse this author
Fukuhara, Takasuke Browse this author →KAKEN DB
Keywords: RNA virus
liver transplantation
RNA sequencing
Issue Date: Jul-2021
Publisher: MDPI
Journal Title: Viruses-Basel
Volume: 13
Issue: 7
Start Page: 1310
Publisher DOI: 10.3390/v13071310
Abstract: Although viruses infect various organs and are associated with diseases, there may be many unidentified pathogenic viruses. The recent development of next-generation sequencing technologies has facilitated the establishment of an environmental viral metagenomic approach targeting the intracellular viral genome. However, an efficient method for the detection of a viral genome derived from an RNA virus in animal or human samples has not been established. Here, we established a method for the efficient detection of RNA viruses in human clinical samples. We then tested the efficiency of the method compared to other conventional methods by using tissue samples collected from 57 recipients of living donor liver transplantations performed between June 2017 and February 2019 at Kyushu University Hospital. The viral read ratio in human clinical samples was higher by the new method than by the other conventional methods. In addition, the new method correctly identified viral RNA from liver tissues infected with hepatitis C virus. This new technique will be an effective tool for intracellular RNA virus surveillance in human clinical samples and may be useful for the detection of new RNA viruses associated with diseases.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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