| Title: | DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples |
| Authors: | Izumi, Takuma Browse this author |
| Morioka, Yuhei Browse this author |
| Urayama, Syun-ichi Browse this author |
| Motooka, Daisuke Browse this author |
| Tamura, Tomokazu Browse this author |
| Kawagishi, Takahiro Browse this author |
| Kanai, Yuta Browse this author |
| Kobayashi, Takeshi Browse this author |
| Ono, Chikako Browse this author |
| Morinaga, Akinari Browse this author |
| Tomiyama, Takahiro Browse this author |
| Iseda, Norifumi Browse this author |
| Kosai, Yukiko Browse this author |
| Inokuchi, Shoichi Browse this author |
| Nakamura, Shota Browse this author |
| Tanaka, Tomohisa Browse this author |
| Moriishi, Kohji Browse this author |
| Kariwa, Hiroaki Browse this author |
| Yoshizumi, Tomoharu Browse this author |
| Mori, Masaki Browse this author |
| Matsuura, Yoshiharu Browse this author |
| Fukuhara, Takasuke Browse this author →KAKEN DB |
| Keywords: | RNA virus |
| dsRNA |
| liver transplantation |
| RNA sequencing |
| Issue Date: | Jul-2021 |
| Publisher: | MDPI |
| Journal Title: | Viruses-Basel |
| Volume: | 13 |
| Issue: | 7 |
| Start Page: | 1310 |
| Publisher DOI: | 10.3390/v13071310 |
| Abstract: | Although viruses infect various organs and are associated with diseases, there may be many unidentified pathogenic viruses. The recent development of next-generation sequencing technologies has facilitated the establishment of an environmental viral metagenomic approach targeting the intracellular viral genome. However, an efficient method for the detection of a viral genome derived from an RNA virus in animal or human samples has not been established. Here, we established a method for the efficient detection of RNA viruses in human clinical samples. We then tested the efficiency of the method compared to other conventional methods by using tissue samples collected from 57 recipients of living donor liver transplantations performed between June 2017 and February 2019 at Kyushu University Hospital. The viral read ratio in human clinical samples was higher by the new method than by the other conventional methods. In addition, the new method correctly identified viral RNA from liver tissues infected with hepatitis C virus. This new technique will be an effective tool for intracellular RNA virus surveillance in human clinical samples and may be useful for the detection of new RNA viruses associated with diseases. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82551 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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