| Title: | One year exposure to Cd- and Pb-contaminated soil causes metal accumulation and alteration of global DNA methylation in rats |
| Authors: | Nakayama, Shouta M. M. Browse this author |
| Nakata, Hokuto Browse this author |
| Ikenaka, Yoshinori Browse this author |
| Yabe, John Browse this author |
| Oroszlany, Balazs Browse this author |
| Yohannes, Yared B. Browse this author |
| Bortey-Sam, Nesta Browse this author |
| Muzandu, Kaampwe Browse this author |
| Choongo, Kennedy Browse this author |
| Kuritani, Takeshi Browse this author |
| Nakagawa, Mitsuhiro Browse this author |
| Ishizuka, Mayumi Browse this author →KAKEN DB |
| Keywords: | Cd |
| DNA methylation |
| Epigenetics |
| Pb isotope |
| Soil exposure |
| Issue Date: | Sep-2019 |
| Publisher: | Elsevier |
| Journal Title: | Environmental Pollution |
| Volume: | 252 |
| Start Page: | 1267 |
| End Page: | 1276 |
| Publisher DOI: | 10.1016/j.envpol.2019.05.038 |
| Abstract: | Metal pollution has been associated with anthropogenic activities, such as effluents and emissions from mines. Soil could be exposure route of wild rats to metals, especially in mining areas. The aim of this study was to verify whether soil exposure under environmentally relevant circumstances results in metal accumulation and epigenetic modifications. Wistar rats were divided to three groups: 1) control without soil exposure, 2) low-metal exposure group exposed to soil containing low metal levels (Pb: 75 mg/kg; Cd: 0.4), and 3) high-metal exposure group exposed to soil (Pb: 3750; Cd: 6). After 1 year of exposure, the metal levels, Pb isotopic values, and molecular indicators were measured. Rats in the high-group showed significantly greater concentrations of Pb and Cd in tissues. Higher accumulation factors (tissue/soil) of Cd than Pb were observed in the liver, kidney, brain, and lung, while the factor of Pb was higher in the tibia. The obtained results of metal accumulation ratios (lung/liver) and stable Pb isotope ratios in the tissues indicated that the respiratory exposure would account for an important share of metal absorption into the body. Genome-wide methylation status and DNA methyltransferase (Dnmt 3a/3b) mRNA expressions in testis were higher in the high-group, suggesting that exposure to soil caused metal accumulation and epigenetic alterations in rats. (C) 2019 Elsevier Ltd. All rights reserved. |
| Rights: | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/82568 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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