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One year exposure to Cd- and Pb-contaminated soil causes metal accumulation and alteration of global DNA methylation in rats

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Title: One year exposure to Cd- and Pb-contaminated soil causes metal accumulation and alteration of global DNA methylation in rats
Authors: Nakayama, Shouta M. M. Browse this author
Nakata, Hokuto Browse this author
Ikenaka, Yoshinori Browse this author
Yabe, John Browse this author
Oroszlany, Balazs Browse this author
Yohannes, Yared B. Browse this author
Bortey-Sam, Nesta Browse this author
Muzandu, Kaampwe Browse this author
Choongo, Kennedy Browse this author
Kuritani, Takeshi Browse this author
Nakagawa, Mitsuhiro Browse this author
Ishizuka, Mayumi Browse this author →KAKEN DB
Keywords: Cd
DNA methylation
Pb isotope
Soil exposure
Issue Date: Sep-2019
Publisher: Elsevier
Journal Title: Environmental Pollution
Volume: 252
Start Page: 1267
End Page: 1276
Publisher DOI: 10.1016/j.envpol.2019.05.038
Abstract: Metal pollution has been associated with anthropogenic activities, such as effluents and emissions from mines. Soil could be exposure route of wild rats to metals, especially in mining areas. The aim of this study was to verify whether soil exposure under environmentally relevant circumstances results in metal accumulation and epigenetic modifications. Wistar rats were divided to three groups: 1) control without soil exposure, 2) low-metal exposure group exposed to soil containing low metal levels (Pb: 75 mg/kg; Cd: 0.4), and 3) high-metal exposure group exposed to soil (Pb: 3750; Cd: 6). After 1 year of exposure, the metal levels, Pb isotopic values, and molecular indicators were measured. Rats in the high-group showed significantly greater concentrations of Pb and Cd in tissues. Higher accumulation factors (tissue/soil) of Cd than Pb were observed in the liver, kidney, brain, and lung, while the factor of Pb was higher in the tibia. The obtained results of metal accumulation ratios (lung/liver) and stable Pb isotope ratios in the tissues indicated that the respiratory exposure would account for an important share of metal absorption into the body. Genome-wide methylation status and DNA methyltransferase (Dnmt 3a/3b) mRNA expressions in testis were higher in the high-group, suggesting that exposure to soil caused metal accumulation and epigenetic alterations in rats. (C) 2019 Elsevier Ltd. All rights reserved.
Rights: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石塚 真由美

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