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Selection of reference genes for quantitative PCR analysis in poultry red mite (Dermanyssus gallinae)

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Title: Selection of reference genes for quantitative PCR analysis in poultry red mite (Dermanyssus gallinae)
Authors: Ariizumi, Takuma Browse this author
Murata, Shiro Browse this author →KAKEN DB
Fujisawa, Sotaro Browse this author
Isezaki, Masayoshi Browse this author
Maekawa, Naoya Browse this author →KAKEN DB
Okagawa, Tomohiro Browse this author →KAKEN DB
Sato, Takumi Browse this author
Oishi, Eiji Browse this author
Taneno, Akira Browse this author
Konnai, Satoru Browse this author →KAKEN DB
Ohashi, Kazuhiko Browse this author →KAKEN DB
Keywords: apolipophorins
Dermanyssus gallinae
Elongation factor 1-alpha
poultry red mite
reference genes
Issue Date: 9-Apr-2021
Publisher: 公益社団法人 日本獣医学会 (The Japanese Society of Veterinary Science)
Journal Title: Journal of veterinary medical science
Volume: 83
Issue: 4
Start Page: 558
End Page: 565
Publisher DOI: 10.1292/jvms.20-0677
Abstract: Poultry red mites (PRMs, Dermanyssus gallinae) are harmful ectoparasites that affect farmed chickens and cause serious economic losses in the poultry industry worldwide. Acaricides are used for PRM control; however, some PRMs have developed acaricide-resistant properties, which have indicated the need for different approaches for PRM control.Therefore, it is necessary to elucidate the biological status of PRMs to develop alternative PRM control strategies. Quantitative polymerase chain reaction (qPCR) allows analysis of the biological status at the transcript level. However, reference genes are preferable for accurate comparison of expression level changes given the large variation in the quality of the PRM samples collected in each farm. This study aimed to identify candidate reference genes with stable expression levels in the different blood feeding states and life stages of PRMs. First, we selected candidates based on the following criteria: sufficient expression intensity and no significant expression difference between fed and starved states. We selected and characterized seven candidate reference genes. Among them, we evaluated the gene expression stability between the starved and fed states using RefFinder; moreover, we compared their expression levels in each life-stage and identified two reference genes, Elongation factor 1-alpha (ELF1A)-like and apolipophorins-like. Finally, we evaluated the utility of the candidates as reference genes, and the use of ELF1A-like and apolipophorins-like successfully normalized ATP synthase subunit g -like gene expression. Thus, ELF1A-like and apolipophorins-like could be suitable reference genes in PRMs.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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