| Title: | Glucokinase is required for high-starch diet-induced beta-cell mass expansion in mice |
| Authors: | Tsuchida, Kazuhisa Browse this author |
| Nakamura, Akinobu Browse this author →KAKEN DB |
| Miyoshi, Hideaki Browse this author |
| Yang, Kelaier Browse this author |
| Yamauchi, Yuki Browse this author |
| Kawata, Shinichiro Browse this author |
| Omori, Kazuno Browse this author |
| Takahashi, Kiyohiko Browse this author |
| Kitao, Naoyuki Browse this author |
| Nomoto, Hiroshi Browse this author |
| Kameda, Hiraku Browse this author |
| Cho, Kyu Yong Browse this author |
| Seino, Yusuke Browse this author |
| Terauchi, Yasuo Browse this author |
| Atsumi, Tatsuya Browse this author |
| Keywords: | β-Cell mass |
| Glucokinase |
| High-starch diet |
| Issue Date: | Sep-2021 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Journal of diabetes investigation |
| Volume: | 12 |
| Issue: | 9 |
| Start Page: | 1545 |
| End Page: | 1554 |
| Publisher DOI: | 10.1111/jdi.13532 |
| Abstract: | Aims/Introduction We aimed to determine whether glucokinase is required for beta-cell mass expansion induced by high-starch diet (HSTD)-feeding, as has been shown in its high-fat diet-induced expansion. Materials and Methods Eight-week-old male wild-type (Gck(+/+)) or glucokinase haploinsufficient (Gck(+/-)) mice were fed either a normal chow (NC) or an HSTD for 15 weeks. The bodyweight, glucose tolerance, insulin sensitivity, insulin secretion and beta-cell mass were assessed. Results Both HSTD-fed Gck(+/+) and Gck(+/-) mice had significantly higher bodyweight than NC-fed mice. Insulin and oral glucose tolerance tests revealed that HSTD feeding did not affect insulin sensitivity nor glucose tolerance in either the Gck(+/+) or Gck(+/-) mice. However, during the oral glucose tolerance test, the 15-min plasma insulin concentration after glucose loading was significantly higher in the HSTD group than that in the NC group for Gck(+/+), but not for Gck(+/-) mice. beta-Cell mass was significantly larger in HSTD-fed Gck(+/+) mice than that in NC-fed Gck(+/+) mice. In contrast, the beta-cell mass of the HSTD-fed Gck(+/-) mice was not different from that of the NC-fed Gck(+/-) mice. Conclusions The results showed that HSTD feeding would increase pancreatic beta-cell mass and insulin secretion in Gck(+/+), but not Gck(+/-) mice. This observation implies that glucokinase in beta-cells would be required for the increase in beta-cell mass induced by HSTD feeding. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82778 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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