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Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles

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Title: Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles
Authors: Furihata, Tomokazu Browse this author
Miyaji, Hirofumi Browse this author →KAKEN DB
Nishida, Erika Browse this author →KAKEN DB
Kato, Akihito Browse this author →KAKEN DB
Miyata, Saori Browse this author
Shitomi, Kanako Browse this author
Mayumi, Kayoko Browse this author
Kanemoto, Yukimi Browse this author
Sugaya, Tsutomu Browse this author →KAKEN DB
Akasaka, Tsukasa Browse this author →KAKEN DB
Keywords: bone filling material
integrin β 1
osteogenic differentiation
rat skull
recombinant peptide based on human collagen type I
Issue Date: Oct-2020
Publisher: John Wiley & Sons
Journal Title: Journal of biomedical materials research. Part B, Applied biomaterials
Volume: 108
Issue: 7
Start Page: 3033
End Page: 3044
Publisher DOI: 10.1002/jbm.b.34632
PMID: 32386261
Abstract: Recombinant human collagen peptide, developed based on human collagen type I, contains an arginyl-glycyl-aspartic acid (RGD)-rich motif to enhance cell behavior and is anticipated as a xeno-free polymer material for use in tissue engineering. We fabricated granules containing recombinant human collagen peptide (RCP) applied with beta-tricalcium phosphate fine particles (RCP/β-TCP) as bone filling scaffold material and assessed the bone forming ability of RCP/β-TCP. Recombinant peptide was thermal crosslinked and freeze-dried to prepare RCP. An aqueous dispersion of β-TCP fine particles was added to RCP to obtain RCP/β-TCP. Subsequently, RCP/β-TCP were characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDX), and cell culture assessments. Furthermore, RCP/β-TCP were implanted into rat cranial bone defects for radiographic and histological evaluations. In SEM and EDX analyses of RCP/β-TCP, β-TCP particles dose-dependently covered the surface of RCP. Cell culture tests showed that RCP/β-TCP remarkably promoted proliferation and mRNA expression of various genes, such as integrin β1 and osteogenic markers, of osteoblastic MC3T3-E1 cells. Histomorphometric assessment at 4 weeks showed that RCP/β-TCP significantly promoted new skull bone formation compared to RCP (p < 0.05) and control (no application) (p < 0.01). Accordingly, these findings suggest RCP/β-TCP possess bone forming capability and would be beneficial for bone tissue engineering therapy.
Rights: This is the peer reviewed version of the following article: [Furihata T, Miyaji H, Nishida E, et al. Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles. J Biomed Mater Res B Appl Biomater. 2020;10.1002/jbm.b.34632.], which has been published in final form at []. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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