| Title: | Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles |
| Authors: | Furihata, Tomokazu Browse this author |
| Miyaji, Hirofumi Browse this author →KAKEN DB |
| Nishida, Erika Browse this author →KAKEN DB |
| Kato, Akihito Browse this author →KAKEN DB |
| Miyata, Saori Browse this author |
| Shitomi, Kanako Browse this author |
| Mayumi, Kayoko Browse this author |
| Kanemoto, Yukimi Browse this author |
| Sugaya, Tsutomu Browse this author →KAKEN DB |
| Akasaka, Tsukasa Browse this author →KAKEN DB |
| Keywords: | bone filling material |
| integrin β 1 |
| osteogenic differentiation |
| rat skull |
| recombinant peptide based on human collagen type I |
| Issue Date: | Oct-2020 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Journal of biomedical materials research. Part B, Applied biomaterials |
| Volume: | 108 |
| Issue: | 7 |
| Start Page: | 3033 |
| End Page: | 3044 |
| Publisher DOI: | 10.1002/jbm.b.34632 |
| PMID: | 32386261 |
| Abstract: | Recombinant human collagen peptide, developed based on human collagen type I, contains an arginyl-glycyl-aspartic acid (RGD)-rich motif to enhance cell behavior and is anticipated as a xeno-free polymer material for use in tissue engineering. We fabricated granules containing recombinant human collagen peptide (RCP) applied with beta-tricalcium phosphate fine particles (RCP/β-TCP) as bone filling scaffold material and assessed the bone forming ability of RCP/β-TCP. Recombinant peptide was thermal crosslinked and freeze-dried to prepare RCP. An aqueous dispersion of β-TCP fine particles was added to RCP to obtain RCP/β-TCP. Subsequently, RCP/β-TCP were characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDX), and cell culture assessments. Furthermore, RCP/β-TCP were implanted into rat cranial bone defects for radiographic and histological evaluations. In SEM and EDX analyses of RCP/β-TCP, β-TCP particles dose-dependently covered the surface of RCP. Cell culture tests showed that RCP/β-TCP remarkably promoted proliferation and mRNA expression of various genes, such as integrin β1 and osteogenic markers, of osteoblastic MC3T3-E1 cells. Histomorphometric assessment at 4 weeks showed that RCP/β-TCP significantly promoted new skull bone formation compared to RCP (p < 0.05) and control (no application) (p < 0.01). Accordingly, these findings suggest RCP/β-TCP possess bone forming capability and would be beneficial for bone tissue engineering therapy. |
| Rights: | This is the peer reviewed version of the following article: [Furihata T, Miyaji H, Nishida E, et al. Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles. J Biomed Mater Res B Appl Biomater. 2020;10.1002/jbm.b.34632.], which has been published in final form at [https://doi.org/10.1002/jbm.b.34632]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/82818 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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