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Radiation-induced abnormal centrosome amplification and mitotic catastrophe in human cervical tumor HeLa cells and murine mammary tumor EMT6 cells
| Title: | Radiation-induced abnormal centrosome amplification and mitotic catastrophe in human cervical tumor HeLa cells and murine mammary tumor EMT6 cells |
| Authors: | Fujimoto, Masaki Browse this author | | Bo, Tomoki Browse this author | | Yamamoto, Kumiko Browse this author | | Yasui, Hironobu Browse this author →KAKEN DB | | Yamamori, Tohru Browse this author →KAKEN DB | | Inanami, Osamu Browse this author →KAKEN DB |
| Keywords: | mitotic catastrophe | | centrosome | | centrinone-B | | radiation | | polo-like kinase 4 (PLK4) |
| Issue Date: | Nov-2020 |
| Publisher: | International Press of Boston |
| Journal Title: | Journal of Clinical Biochemistry and Nutrition (JCBN) |
| Volume: | 67 |
| Issue: | 3 |
| Start Page: | 240 |
| End Page: | 247 |
| Publisher DOI: | 10.3164/jcbn.19-80 |
| Abstract: | Mitotic catastrophe is a form of cell death linked to aberrant mitosis caused by improper or uncoordinated mitotic progression. Abnormal centrosome amplification and mitotic catastrophe occur simultaneously, and some cells with amplified centrosomes enter aberrant mitosis, but it is not clear whether abnormal centrosome amplification triggers mitotic catastrophe. Here, to investigate whether radiation-induced abnormal centrosome amplification is essential for induction of radiation-induced mitotic catastrophe, centrinone-B, a highly selective inhibitor of polo-like kinase 4, was utilized to inhibit centrosome amplification, since polo-like kinase 4 is an essential kinase in centrosome duplication. When human cervical tumor HeLa cells and murine mammary tumor EMT6 cells were irradiated with 2.5 Gy of X-rays, cells with morphological features of mitotic catastrophe and the number of cells having >2 centrosomes increased in both cell lines. Although centrinone-B significantly inhibited radiation-induced abnormal centrosome amplification in both cell lines, such treatment did not change cell growth and significantly enhanced mitotic catastrophe in HeLa cells exposed to X-rays. In contrast, inhibition of centrosome amplification reduced cell growth and mitotic catastrophe in EMT6 cells exposed to X-rays. These results indicated that the role of radiation -induced abnormal centrosome amplification in radiation induced mitotic catastrophe changes, depending on the cell type. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82881 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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