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Wild ciliates differ in susceptibility to Legionella pneumophila JR32

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Title: Wild ciliates differ in susceptibility to Legionella pneumophila JR32
Authors: Kawashiro, Airi Browse this author
Okubo, Torahiko Browse this author →KAKEN DB
Nakamura, Shinji Browse this author
Thapa, Jeewan Browse this author →KAKEN DB
Miyake, Masaki Browse this author
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Keywords: Anteglaucoma CS11A
Colpoda E6
Legionella pneumophila JR32
Issue Date: Aug-2021
Publisher: Society for General Microbiology
Journal Title: Microbiology-SGM
Volume: 167
Issue: 8
Start Page: 001078
Publisher DOI: 10.1099/mic.0.001078
Abstract: We investigated how Legionella pneumophila (Lp) JR32 interacts with Anteglaucoma CS11A and Colpoda E6, two ciliates that we isolated from sewage and sink trap sludge, respectively, using a handmade maze device containing a 96-well crafting plate. Our 185 rDNA-based phylogenetic analysis showed that Anteglaucoma CS11A and Colpoda E6 formed distinct clades. Scanning electron microscopy showed that Anteglaucoma CS11A had a bigger-sized body than Colpoda E6 and, unlike Tetrahymena IB (the reference strain), neither ciliate produced pellets, which are extracellular vacuoles. Fluorescence microscopic observations revealed that although the intake amounts differed, all three ciliates rapidly ingested LpJR32 regardless of the presence or absence of the icm/dot virulence genes, indicating that they all interacted with LpJR32. In co-cultures with Anteglaucoma CS11A, the LpJR32 levels were maintained but fell dramatically when the co-culture contained the LpJR32 icm/dot deletion mutant instead. Anteglaucoma CS11 A died within 2days of co-culture with LpJR32, but survived co-culture with the deletion mutant. In co-cultures with Colpoda E6, LpJR32 levels were maintained but temporarily decreased independently of the virulence gene. Concurrently, the Colpoda E6 ciliates survived by forming cysts, which may enable them to resist harsh environments, and by diminishing the sensitivity of trophozoites to Lp. In the Tetrahymena IB co-cultures with LpJR32 or Delta icm/dot, the Lp levels were maintained, albeit with temporal decreases, and the Tetrahymena IB levels were also maintained. We conclude that unlike Tetrahymena IB with pellet production, Anteglaucoma CS11A can be killed by LpJR32 infection, and Colpoda E6 can resist LpJR32 infection through cyst formation and the low sensitivity of trophozoites to Lp. Thus, the two ciliates that we isolated had different susceptibilities to LpJR32 infection.
Rights: © Kawashiro Airi, Okubo Torahiko, Nakamura Shinji, Thapa Jeewan, Miyake Masaki, Yamaguchi Hiroyuki, 2021. The definitive peer reviewed, edited version of this article is published in Microbiology-SGM, 167, 8, 2021, 10.1099/mic.0.001078.
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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