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Hokkaido University Collection of Scholarly and Academic Papers >
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Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway
| Title: | Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway |
| Authors: | Suzuki, Yukari1 Browse this author | | Ohgami, Kazuhiro Browse this author →KAKEN DB | | Shiratori, Kenji Browse this author | | Jin, Xue-Hai Browse this author | | Ilieva, Iliyana Browse this author | | Koyama, Yoshikazu Browse this author | | Yazawa, Kazunaga Browse this author | | Yoshida, Kazuhiko Browse this author →KAKEN DB | | Kase, Satoru Browse this author →KAKEN DB | | Ohno, Shigeaki10 Browse this author →KAKEN DB |
| Authors(alt): | 鈴木, 由香里1 | | 大野, 重昭10 |
| Keywords: | endotoxin-induced uveitis | | anti-inflammation agent | | NF-κB |
| Issue Date: | Feb-2006 |
| Publisher: | Elsevier Ltd. |
| Journal Title: | Experimental Eye Research |
| Volume: | 82 |
| Issue: | 2 |
| Start Page: | 275 |
| End Page: | 281 |
| Publisher DOI: | 10.1016/j.exer.2005.06.023 |
| PMID: | 16126197 |
| Abstract: | We investigated the effects of astxanthin (AST), a carotenoid, on endotoxin-induced uveitis (EIU), and measured the expression of inflammatory cytokines and chemokines in the presence or absence of AST over the course of the disease. EIU was induced in male Lewis rats by footpad injection of lipopolysaccharide (LPS). Immediately after the LPS inoculation, either 1, 10, or 100 mg/kg of AST were injected intravenously. Aqueous humor was collected at 6, 12 and 24 hours after LPS inoculation and the number of infiltrating cells in the anterior chamber were counted. In addition, we assayed the concentration of protein, nitric oxide (NO), tumor necrosis factor (TNF)- and prostaglandin (PG) E2. Immunohistochemical staining with a monoclonal antibody against activated NF-B was performed in order to evaluate the effects of AST on NF-B activation. Rats injected with AST showed a significant decrease in the number of infiltrating cells in anterior chamber. Moreover, AST-treated rats with EIU showed significantly lower concentrations of protein, NO, TNF- and PGE2 in the aqueous humor. Even the early stages of EIU were suppressed by injection of AST. The number of activated NF-B-positive cells was lower in iris-ciliary bodies treated with 10 or 100 mg/kg AST at 3 hours after LPS injection. These results suggest that AST reduces ocular inflammation in eyes with EIU by downregulating proinflammatory factors and by inhibiting the NF-B-dependent signaling pathway. |
| Relation: | http://www.sciencedirect.com/science/journal/00144835 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/8292 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 大野 重昭
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