| Title: | Genomic profiling of intestinal/mixed-type superficial non-ampullary duodenal epithelial tumors |
| Authors: | Miyamoto, Shuichi Browse this author |
| Suda, Goki Browse this author →KAKEN DB |
| Ishikawa, Marin Browse this author |
| Hayashi, Hideyuki Browse this author |
| Nimura, Satoshi Browse this author |
| Matsuno, Yoshihiro Browse this author |
| Mori, Ryo Browse this author |
| Tanishima, Shigeki Browse this author |
| Kudo, Takahiko Browse this author |
| Takagi, Tomofumi Browse this author |
| Yamamoto, Yoshiya Browse this author |
| Ono, Shoko Browse this author |
| Shimizu, Yuichi Browse this author |
| Sakamoto, Naoya Browse this author →KAKEN DB |
| Keywords: | duodenal cancer |
| genomic testing |
| next-generation sequencing |
| superficial non-ampullary duodenal epithelial tumor |
| Issue Date: | Sep-2021 |
| Publisher: | John Wiley & Sons |
| Journal Title: | JGH open |
| Volume: | 5 |
| Issue: | 9 |
| Start Page: | 1071 |
| End Page: | 1077 |
| Publisher DOI: | 10.1002/jgh3.12632 |
| Abstract: | Background and Aim: The mechanism underlying carcinogenesis and the genomic features of superficial non-ampullary duodenal epithelial tumors (SNADETs) have not been elucidated in detail. In this study, we examined the genomic features of incipient SNADETs, such as small lesions resected via endoscopic treatment, using next-generation sequencing (NGS). Methods: Twenty consecutive patients who underwent endoscopic treatment for SNADETs of less than 20 mm between January and December 2017 were enrolled. Targeted genomic sequencing was performed through NGS using a panel of 160 cancer-related genes. Furthermore, the alteration/mutation frequencies in SNADETs were examined. Results: The maximum size of the SNADETs examined in this study was 12 mm in diameter. Five SNADETs were classified as low-grade dysplasia (LGD) tumors, while 14 SNADETs were classified as high-grade dysplasia tumors. Only one carcinoma in situ was detected. NGS data for 16 samples were obtained. APC alterations were detected in 81% of samples (13/16). KRAS, BRAF, and TP53 alterations were detected in 25% (4/16), 18.8% (3/16), and 6.3% (1/16) of cases, respectively. Conclusion: We detected APC alterations in most small SNADETs resected via endoscopic treatment, from LGD to carcinoma samples. Even in SNADETs classified as small LGD exhibited KRAS and BRAF alterations. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82978 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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