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Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/8299

Title: Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland
Authors: Yamaji, Daisuke Browse this author
Kimura, Kazuhiro Browse this author →KAKEN DB
Watanabe, Atsushi Browse this author
Kon, Yasuhiro Browse this author →KAKEN DB
Iwanaga, Toshihiko Browse this author →KAKEN DB
Soliman, Mohamed M. Browse this author
Ahmed, Mohamed M. Browse this author
Saito, Masayuki Browse this author
Keywords: HGF
c-Met
cDNA cloning
mammary gland
epithelial cells
Issue Date: Mar-2006
Publisher: Elsevier
Journal Title: Domestic Animal Endocrinology
Volume: 30
Issue: 3
Start Page: 239
End Page: 246
Publisher DOI: 10.1016/j.domaniend.2005.08.005
PMID: 16207523
Abstract: Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic cytokine that plays a crucial role in the embryonic and postnatal development of various organs including the mammary gland. We cloned bovine HGF and its c-Met receptor cDNAs, and examined their expression during mammary gland development in dairy cows. The 2.5-kbp HGF cDNA clone contained a 2,190 bp open reading frame coding a 730 amino acid protein, while the 4.8-kbp c-Met cDNA clone contained a 4,152 bp open reading frame coding a 1,384 amino acid protein. The bovine HGF and c-Met sequences exhibited more than 87% identity with those of other mammals. RT-PCR analysis revealed ubiquitous expression of both HGF and c-Met mRNAs in various bovine tissues tested. HGF mRNA was detected only in the inactive stage of bovine mammary gland development and not in the developing, lactating, and involuting stages, while c-Met mRNA was detected in the inactive and involuting stages. Immunohistochemical analysis demonstrated that the c-Met protein was found on mammary epithelial cells in the inactive, developing, and involuting stages, and on myoepithelial cells in all stages. These results suggest pivotal roles of HGF and c-Met in the development of bovine mammary gland.
Description URI: http://www.sciencedirect.com/science/journal/07397240
Type: article (author version)
URI: http://hdl.handle.net/2115/8299
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 和弘

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