| Title: | Association of neutrophil extracellular traps with the development of idiopathic osteonecrosis of the femoral head |
| Authors: | Nonokawa, Mayu Browse this author |
| Shimizu, Tomohiro Browse this author →KAKEN DB |
| Yoshinari, Miku Browse this author |
| Hashimoto, Yamato Browse this author |
| Nakamura, Yusuke Browse this author |
| Takahashi, Daisuke Browse this author →KAKEN DB |
| Asano, Tsuyoshi Browse this author →KAKEN DB |
| Nishibata, Yuka Browse this author |
| Masuda, Sakiko Browse this author →KAKEN DB |
| Nakazawa, Daigo Browse this author →KAKEN DB |
| Tanaka, Satoshi Browse this author →KAKEN DB |
| Tomaru, Utano Browse this author →KAKEN DB |
| Iwasaki, Norimasa Browse this author →KAKEN DB |
| Ishizu, Akihiro Browse this author →KAKEN DB |
| Issue Date: | Nov-2020 |
| Publisher: | Elsevier |
| Journal Title: | The American Journal of Pathology |
| Volume: | 190 |
| Issue: | 11 |
| Start Page: | 2282 |
| End Page: | 2289 |
| Publisher DOI: | 10.1016/j.ajpath.2020.07.008 |
| PMID: | 32702358 |
| Abstract: | Idiopathic osteonecrosis of the femoral head (ONFH) is defined as necrosis of osteocytes due to a non-traumatic ischemia of the femoral head. Iatrogenic glucocorticoid administration and habitual alcohol intake are regarded as risk factors. It has been suggested that glucocorticoid-induced activation of platelets contribute to the local blood flow disturbance of the femoral head. Both activated platelets and alcohol can induce neutrophil extracellular traps (NETs). To determine the association of NETs with the development of idiopathic ONFH, surgically resected femoral heads of patients with idiopathic ONFH and osteoarthritis (OA) were assessed for existence of NET-forming neutrophils by immunofluorescent staining. As a result, NET-forming neutrophils were present in small vessels surrounding the femoral head of patients with idiopathic ONFH but not OA. Moreover, Wistar-Kyoto rats were intravenously injected with NET-forming neutrophils or neutrophils without NET induction, and then the ischemic state of the tissue around the femoral head was evaluated by immunohistochemistry for hypoxia-inducible factor-1α (HIF-1α). NET-forming neutrophils circulated into the tissue around the femoral head, and HIF-1α expression in the tissue was higher compared to that of rats intravenously administered with neutrophils without NET induction. Furthermore, ischemic change of osteocytes was observed in the femoral head of rats given an intravenous injection of NET-forming neutrophils. The collective findings suggest that NETs are possibly associated with the development of idiopathic ONFH. |
| Rights: | © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/83133 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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