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Utilizing attached hard ticks as pointers to the risk of infection by Babesia and Theileria species in sika deer (Cervus nippon yesoensis), in Japan

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Title: Utilizing attached hard ticks as pointers to the risk of infection by Babesia and Theileria species in sika deer (Cervus nippon yesoensis), in Japan
Authors: Elzahara, Elbaz Browse this author
Mohamed Abdallah Mohamed, Moustafa Browse this author
Kyunglee, Lee Browse this author
Ching, Alice Lau Ching Browse this author
Shimozuru, Michito Browse this author
Sashika, Mariko Browse this author
Nakao, Ryo Browse this author
El-khodery, Sabry Ahmed Browse this author
Tsubota, Toshio Browse this author →KAKEN DB
Keywords: Babesia
Hard ticks
Reverse line blot
Sika deer
Issue Date: Nov-2020
Publisher: Springer
Journal Title: Experimental and Applied Acarology
Volume: 82
Issue: 3
Start Page: 411
End Page: 429
Publisher DOI: 10.1007/s10493-020-00551-w
Abstract: Ticks are hematophagous ectoparasites that have a significant impact on their animal hosts. Along with mosquitoes, they are the main arthropod vectors of disease agents in domestic animals, wildlife and humans. To investigate the occurrence and prevalence of piroplasmids in ticks, DNA was extracted from 519 hard ticks collected from 116 hunted Hokkaido sika deer (Cervus nippon yesoensis). The success of the DNA extraction was confirmed by touchdown PCR targeting the mitochondrial 16S rDNA gene of ticks. Touchdown PCR and reverse line blot (RLB) hybridization targeting the 18S rRNA gene were used to detect 14 piroplasm species. All hard ticks parasitizing Hokkaido sika deer were identified as belonging to the generaIxodesandHaemaphysalis. In total 163 samples (31.4%) were positive forBabesiaandTheileriaspp. among tick species according to RLB hybridization. Tick DNA hybridized to the oligonucleotide probes ofTheileriasp.Thrivae (27.0% of ticks; 140/519),Theileria capreoli(10.6%; 55/519),Babesia divergens-like (1.7%; 9/519),Babesiasp. (Bab-SD) (0.6%; 3/519),Babesia microtiU.S. (0.4%; 2/519), andB. microtiHobetsu (0.4%; 2/519). The partial sequencing and phylogenetic analyses of the 18S rRNA gene confirmed the RLB hybridization results. Further investigations are needed to reveal the epidemiology and respective vectors of these pathogens.
Rights: This is a post-peer-review, pre-copyedit version of an article published in Experimental and Applied Acarology. The final authenticated version is available online at:
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 坪田 敏男

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