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Comparison of cytotoxicity of cigarette smoke extract derived from heat-not-burn and combustion cigarettes in human vascular endothelial cells
| Title: | Comparison of cytotoxicity of cigarette smoke extract derived from heat-not-burn and combustion cigarettes in human vascular endothelial cells |
| Authors: | Horinouchi, Takahiro Browse this author →KAKEN DB | | Miwa, Soichi Browse this author →KAKEN DB |
| Keywords: | Heat-not-burn cigarette | | Combustion cigarette | | Cigarette smoke extract | | Endothelial nitric oxide synthase | | Oxidative stress |
| Issue Date: | Nov-2021 |
| Publisher: | Japanese Pharmacological Society |
| Journal Title: | Journal of pharmacological sciences |
| Volume: | 147 |
| Issue: | 3 |
| Start Page: | 223 |
| End Page: | 233 |
| Publisher DOI: | 10.1016/j.jphs.2021.07.005 |
| Abstract: | The present study compared the properties of mainstream smoke generated from heat-not-burn (HNB) cigarettes and a combustion cigarette (hi-lite (TM) brand). Three types of cigarette heating devices were used to generate cigarette smoke at different heating temperatures [Ploom S (TM) (200 degrees C), glo (TM) (240 degrees C), and IQOS (TM) (300-350 degrees C)]. Mainstream smoke was generated using the following puffing regimen: volume, 55 mL; duration, 3 s; and interval, 30 s. The rank order of particulate phase (nicotine and tar) amounts trapped on a Cambridge filter was Ploom S < glo < IQOS < hi-lite. Heated cigarette-derived smoke extract (hCSE) from the devices except for Ploom S, and burned CSE (bCSE) decreased mitochondria) metabolic activity (glo < IQOS < hi-lite) in human vascular endothelial cells. Furthermore, the cytotoxicity was reduced by removing the particulate phase from the mainstream smoke. Endothelial nitric oxide synthase activity was reduced by nicotine- and tar-free CSE of IQOS and hi-lite (IQOS < hi-lite), but not Ploom S and glo. These inhibitory effects were diminished by removing the carbonyl compounds from the mainstream smoke. These results indicated that the cytotoxicity of hCSE was lower than that of bCSE in vascular endothelial cells. (C) 2021 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/83189 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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