|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Histone chaperone-mediated co-expression assembly of tetrasomes and nucleosomes
| Title: | Histone chaperone-mediated co-expression assembly of tetrasomes and nucleosomes |
| Authors: | Okimune, Kei-ichi Browse this author | | Hataya, Shogo Browse this author | | Matsumoto, Kazuki Browse this author | | Ushirogata, Kanako Browse this author | | Banko, Petra Browse this author | | Takeda, Seiji Browse this author | | Takasuka, Taichi E. Browse this author →KAKEN DB |
| Keywords: | nucleosome | | tetrasome | | NAP1 | | wheat germ cell-free synthesis | | AFM |
| Issue Date: | Nov-2021 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Febs open bio |
| Volume: | 11 |
| Issue: | 11 |
| Start Page: | 2912 |
| End Page: | 2920 |
| Publisher DOI: | 10.1002/2211-5463.13311 |
| Abstract: | The nucleosome, a basic unit of chromatin found in all eukaryotes, is thought to be assembled through the orchestrated activity of several histone chaperones and chromatin assembly factors in a stepwise manner, proceeding from tetrasome assembly, to H2A/H2B deposition, and finally to formation of the mature nucleosome. In this study, we demonstrate chaperone-mediated assembly of both tetrasomes and nucleosomes on the well-defined Widom 601 positioning sequence using a co-expression/reconstitution wheat germ cell-free system. The purified tetrasomes and nucleosomes were positioned around the center of a given sequence. The heights and diameters were measured by atomic force microscopy. Together with the reported unmodified native histones produced by the wheat germ cell-free platform, our method is expected to be useful for downstream applications in the field of chromatin research. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/83316 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
