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Histone chaperone-mediated co-expression assembly of tetrasomes and nucleosomes

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Title: Histone chaperone-mediated co-expression assembly of tetrasomes and nucleosomes
Authors: Okimune, Kei-ichi Browse this author
Hataya, Shogo Browse this author
Matsumoto, Kazuki Browse this author
Ushirogata, Kanako Browse this author
Banko, Petra Browse this author
Takeda, Seiji Browse this author
Takasuka, Taichi E. Browse this author →KAKEN DB
Keywords: nucleosome
wheat germ cell-free synthesis
Issue Date: Nov-2021
Publisher: John Wiley & Sons
Journal Title: Febs open bio
Volume: 11
Issue: 11
Start Page: 2912
End Page: 2920
Publisher DOI: 10.1002/2211-5463.13311
Abstract: The nucleosome, a basic unit of chromatin found in all eukaryotes, is thought to be assembled through the orchestrated activity of several histone chaperones and chromatin assembly factors in a stepwise manner, proceeding from tetrasome assembly, to H2A/H2B deposition, and finally to formation of the mature nucleosome. In this study, we demonstrate chaperone-mediated assembly of both tetrasomes and nucleosomes on the well-defined Widom 601 positioning sequence using a co-expression/reconstitution wheat germ cell-free system. The purified tetrasomes and nucleosomes were positioned around the center of a given sequence. The heights and diameters were measured by atomic force microscopy. Together with the reported unmodified native histones produced by the wheat germ cell-free platform, our method is expected to be useful for downstream applications in the field of chromatin research.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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