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Research Note : Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan
| Title: | Research Note : Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan |
| Authors: | Murata, Shiro Browse this author →KAKEN DB | | Yamamoto, Eiji Browse this author | | Sakashita, Natsumi Browse this author | | Maekawa, Naoya Browse this author →KAKEN DB | | Okagawa, Tomohiro Browse this author →KAKEN DB | | Konnai, Satoru Browse this author →KAKEN DB | | Ohashi, Kazuhiko Browse this author →KAKEN DB |
| Keywords: | deletion | | Marek's disease virus | | Meq | | S-Meq | | transactivation |
| Issue Date: | 1-Nov-2021 |
| Publisher: | Elsevier |
| Journal Title: | Poultry Science |
| Volume: | 100 |
| Issue: | 11 |
| Start Page: | 101461 |
| Publisher DOI: | 10.1016/j.psj.2021.101461 |
| Abstract: | Marek's disease virus (MDV) causes malignant lymphomas in chickens (Marek's disease; MD). Although MD has caused significant economic losses to the poultry industry, currently, its occurrence in the field is effectively controlled by vaccination. However, the genetic characteristics of MDV strains have changed, and the poultry industry has experienced MD outbreaks in vaccinated chickens because of enhanced virulence. Meq, an oncoprotein of MDV, is a key transcription factor correlated with the tumorigenesis in MD. Animal experiments using recombinant MDV revealed that distinct polymorphisms in Meq affect the virulence of MDV strains. Meq containing an insertion or deletion is present in some MDV strains. In the 2010s, field strains that encode Meq containing the deletion (S-Meq) were reported. In this study, we characterized the genetic features of S-Meq detected in a Japanese MDV strain and analyzed its transactivation activity to investigate S-Meq's protein function. S-Meq lacked 41 amino acids, and the deletion was at the same position as those observed in other countries. In addition, S-Meq exhibited higher transactivation activity than Meq from other MDV strains circulating in Japan. These results suggest that the deletion in the transactivation domain may enhance the Meq protein's function. Further investigation is needed to clarify whether the deletion in the transactivation domain of Meq affects MDV's virulence. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/83382 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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