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Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia
| Title: | Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia |
| Authors: | Bwalya, Precious Browse this author | | Yamaguchi, Tomoyuki Browse this author | | Solo, Eddie Samuneti Browse this author | | Chizimu, Joseph Yamweka Browse this author | | Mbulo, Grace Browse this author | | Nakajima, Chie Browse this author →KAKEN DB | | Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Keywords: | Mycobacterium tuberculosis | | streptomycin | | multidrug-resistant tuberculosis (MDR-TB) | | spoligotype | | Zambia |
| Issue Date: | Oct-2021 |
| Publisher: | MDPI |
| Journal Title: | Antibiotics-Basel |
| Volume: | 10 |
| Issue: | 10 |
| Start Page: | 1169 |
| Publisher DOI: | 10.3390/antibiotics10101169 |
| Abstract: | Streptomycin (STR) is recommended for the management of multidrug-resistant tuberculosis (MDR-TB). Streptomycin resistance-conferring mutation types and frequency are shown to be influenced by genotypes of circulating strains in a population. This study aimed to characterize the mutations in MDR-TB isolates and examine their relationship with the genotypes in Zambia. A total of 138 MDR-TB isolates stored at the University Teaching Hospital Tuberculosis Reference Laboratory in Zambia were analyzed using spoligotyping and sequencing of STR resistance-associated genes. Streptomycin resistance was observed in 65.9% (91/138) of MDR-TB isolates. Mutations in rpsL, rrs, and gidB accounted for 33%, 12.1%, and 49.5%, respectively. Amino acid substitution K43R in rpsL was strongly associated with the CAS1_Kili genotype (p < 0.0001). The combination of three genes could predict 91.2% of STR resistance. Clustering of isolates based on resistance-conferring mutations and spoligotyping was observed. The clustering of isolates suggests that the increase in STR-resistant MDR-TB in Zambia is largely due to the spread of resistant strains from inadequate treatment. Therefore, rapid detection of STR resistance genetically is recommended before its use in MDR-TB treatment in Zambia.</p> |
| Type: | article |
| URI: | http://hdl.handle.net/2115/83492 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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