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Molecular analysis of streptomycin-resistance associating genes in Mycobacterium tuberculosis isolates from Nepal
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| Title: | Molecular analysis of streptomycin-resistance associating genes in Mycobacterium tuberculosis isolates from Nepal |
| Authors: | Shrestha, Dipti Browse this author | | Maharjan, Bhagwan Browse this author | | Oo, Nan Aye Thida Browse this author | | Isoda, Norikazu Browse this author →KAKEN DB | | Nakajima, Chie Browse this author →KAKEN DB | | Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Keywords: | Mycobacterium tuberculosis | | Streptomycin | | Drug-resistance | | rpsL | | rrs | | gidB |
| Issue Date: | Dec-2020 |
| Publisher: | Elsevier |
| Journal Title: | Tuberculosis |
| Volume: | 125 |
| Start Page: | 101985 |
| Publisher DOI: | 10.1016/j.tube.2020.101985 |
| Abstract: | Mutation in rpsL (encoding ribosomal protein S12), rrs (encoding 16S ribosomal RNA) and gidB (encoding 7-methylguanosine methyltransferase) are associated with resistance to streptomycin (STR), which is used for the treatment of multi-drug resistant tuberculosis (MDR-TB) in Nepal. The aim of our study is to analyze the correlation between mutations in the target genes and STR-resistance in 197 Mycobacterium tuberculosis (MTB) isolates from Nepal. Mutations in rpsL was harbored by 65.9% of isolates, in which the most common mutation in rpsL is caused by K43R (58.8%) and were significantly associated with Beijing genotype (P < 0.001). About 13.2% of isolates harbored mutations in two highly mutable regions of rrs, the 530 loop and the 912 region. About 13.2% of gidB mutants do not show any mutation in rpsL and rrs, which might suggest the role of gidB mutations in STR-resistance in MTB. In addition, 5.6% of isolates do not show any mutations in three genes examined, suggesting the involvement of other mechanism in STR-resistance in MTB. Our findings can be implemented for the establishment of molecular STR-susceptibility testing, in which tuberculosis can be treated with appropriate drugs and can improve control strategies for DR-TB. |
| Rights: | © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/83725 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 定彦
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