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Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia

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Title: Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia
Authors: Harima, Hayato Browse this author →KAKEN DB
Sasaki, Michihito Browse this author →KAKEN DB
Orba, Yasuko Browse this author →KAKEN DB
Okuya, Kosuke Browse this author
Qiu, Yongjin Browse this author →KAKEN DB
Wastika, Christida E. Browse this author
Changula, Katendi Browse this author
Kajihara, Masahiro Browse this author →KAKEN DB
Simulundu, Edgar Browse this author
Yamaguchi, Tomoyuki Browse this author
Eto, Yoshiki Browse this author
Mori-Kajihara, Akina Browse this author
Sato, Akihiko Browse this author
Taniguchi, Satoshi Browse this author
Takada, Ayato Browse this author →KAKEN DB
Saijo, Masayuki Browse this author
Hang'ombe, Bernard M. Browse this author
Sawa, Hirofumi Browse this author →KAKEN DB
Issue Date: Sep-2021
Publisher: PLOS
Journal Title: PLoS neglected tropical diseases
Volume: 15
Issue: 9
Start Page: e0009768
Publisher DOI: 10.1371/journal.pntd.0009768
Abstract: BackgroundPteropine orthoreovirus (PRV) is an emerging bat-borne zoonotic virus that causes severe respiratory illness in humans. Although PRVs have been identified in fruit bats and humans in Australia and Asia, little is known about the prevalence of PRV infection in Africa. Therefore, this study performed an PRV surveillance in fruit bats in Zambia. MethodsEgyptian fruit bats (Rousettus aegyptiacus, n = 47) and straw-colored fruit bats (Eidolon helvum, n = 33) captured in Zambia in 2017-2018 were screened for PRV infection using RT-PCR and serum neutralization tests. The complete genome sequence of an isolated PRV strain was determined by next generation sequencing and subjected to BLAST and phylogenetic analyses. Replication capacity and pathogenicity of the strain were investigated using Vero E6 cell cultures and BALB/c mice, respectively. ResultsAn PRV strain, tentatively named Nachunsulwe-57, was isolated from one Egyptian fruit bat. Serological assays demonstrated that 98% of sera (69/70) collected from Egyptian fruit bats (n = 37) and straw-colored fruit bats (n = 33) had neutralizing antibodies against PRV. Genetic analyses revealed that all 10 genome segments of Nachunsulwe-57 were closely related to a bat-derived Kasama strain found in Uganda. Nachunsulwe-57 showed less efficiency in viral growth and lower pathogenicity in mice than another PRV strain, Miyazaki-Bali/2007, isolated from a patient. ConclusionsA high proportion of Egyptian fruit bats and straw-colored fruit bats were found to be seropositive to PRV in Zambia. Importantly, a new PRV strain (Nachunsulwe-57) was isolated from an Egyptian fruit bat in Zambia, which had relatively weak pathogenicity in mice. Taken together, our findings provide new epidemiological insights about PRV infection in bats and indicate the first isolation of an PRV strain that may have low pathogenicity to humans. Author summaryPteropine orthoreovirus (PRV) is a causative agent of acute respiratory illness in humans in tropical and sub-tropical regions in Southeast Asia. PRVs have been originally isolated from fruit bats, and it is assumed that PRVs spread to humans by both bat-to-human and human-to-human transmission. Recently, an PRV was also detected from a fruit bat in the Afrotropical region and might potentially cause an emerging infection of the bat-borne zoonotic virus in Africa. However, little is known about the prevalence of PRV infection in Africa. In this study, we demonstrated the high prevalence of PRV infection in bat populations in Zambia and isolated a new strain of PRV from Egyptian fruit bats. In addition, we found that the bat-derived PRV strain had lower pathogenicity in mice than a human-derived PRV strain isolated from a patient in Southeast Asia. Our findings provide new epidemiological information about PRV in fruit bats in the Afrotropical region and indicate the first isolation of an PRV strain that may cause attenuated infection in humans.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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