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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Comparative Genomic and Physiological Analysis against Clostridium scindens Reveals Eubacterium sp. c-25 as an Atypical Deoxycholic Acid Producer of the Human Gut Microbiota
| Title: | Comparative Genomic and Physiological Analysis against Clostridium scindens Reveals Eubacterium sp. c-25 as an Atypical Deoxycholic Acid Producer of the Human Gut Microbiota |
| Authors: | Song, Isaiah Browse this author | | Gotoh, Yasuhiro Browse this author | | Ogura, Yoshitoshi Browse this author | | Hayashi, Tetsuya Browse this author | | Fukiya, Satoru Browse this author →KAKEN DB | | Yokota, Atsushi Browse this author →KAKEN DB |
| Keywords: | secondary bile acid | | deoxycholic acid | | bai operon | | gut microbiome | | Clostridium scindens |
| Issue Date: | Nov-2021 |
| Publisher: | MDPI |
| Journal Title: | Microorganisms |
| Volume: | 9 |
| Issue: | 11 |
| Start Page: | 2254 |
| Publisher DOI: | 10.3390/microorganisms9112254 |
| Abstract: | The human gut houses bile acid 7 alpha-dehydroxylating bacteria that produce secondary bile acids such as deoxycholic acid (DCA) from host-derived bile acids through enzymes encoded by the bai operon. While recent metagenomic studies suggest that these bacteria are highly diverse and abundant, very few DCA producers have been identified. Here, we investigated the physiology and determined the complete genome sequence of Eubacterium sp. c-25, a DCA producer that was isolated from human feces in the 1980s. Culture experiments showed a preference for neutral to slightly alkaline pH in both growth and DCA production. Genomic analyses revealed that c-25 is phylogenetically distinct from known DCA producers and possesses a multi-cluster arrangement of predicted bile-acid inducible (bai) genes that is considerably different from the typical bai operon structure. This arrangement is also found in other intestinal bacterial species, possibly indicative of unconfirmed 7 alpha-dehydroxylation capabilities. Functionality of the predicted bai genes was supported by the induced expression of baiB, baiCD, and baiH in the presence of cholic acid substrate. Taken together, Eubacterium sp. c-25 is an atypical DCA producer with a novel bai gene cluster structure that may represent an unexplored genotype of DCA producers in the human gut. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/83814 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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