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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Enhancement of the Immunostimulatory Effect of Phosphodiester CpG Oligodeoxynucleotides by an Antiparallel Guanine-Quadruplex Structural Scaffold
| Title: | Enhancement of the Immunostimulatory Effect of Phosphodiester CpG Oligodeoxynucleotides by an Antiparallel Guanine-Quadruplex Structural Scaffold |
| Authors: | Safitri, Fika Ayu Browse this author | | Tu, Anh Thi Tram Browse this author | | Hoshi, Kazuaki Browse this author | | Shobo, Miwako Browse this author | | Zhao, Dandan Browse this author | | Witarto, Arief Budi Browse this author | | Sumarsono, Sony Heru Browse this author | | Giri-Rachman, Ernawati Arifin Browse this author | | Tsukakoshi, Kaori Browse this author | | Ikebukuro, Kazunori Browse this author | | Yamazaki, Tomohiko Browse this author |
| Keywords: | guanine quadruplex | | high-order structure | | monomeric | | immunostimulatory activity | | adjuvant |
| Issue Date: | Nov-2021 |
| Publisher: | MDPI |
| Journal Title: | Biomolecules |
| Volume: | 11 |
| Issue: | 11 |
| Start Page: | 1617 |
| Publisher DOI: | 10.3390/biom11111617 |
| Abstract: | Guanine-quadruplex-based CpG oligodeoxynucleotides (G4 CpG ODNs) have been developed as potent immunostimulatory agents with reduced sensitivity to nucleases. We designed new monomeric G4 ODNs with an antiparallel topology using antiparallel type duplex/G4 ODNs as robust scaffolds, and we characterized their topology and effects on cytokine secretion. Based on circular dichroism analysis and quantification of mRNA levels of immunostimulatory cytokines, it was found that monomeric antiparallel G4 CpG ODNs containing two CpG motifs in the first functional loop, named G2.0.0, could maintain antiparallel topology and generate a high level of immunostimulatory cytokines in RAW264 mouse macrophage-like cell lines. We also found that the flanking sequence in the CpG motif altered the immunostimulatory effects. Gc2c.0.0 and Ga2c.0.0 are monomeric antiparallel G4 CpG ODNs with one cytosine in the 3 & PRIME; terminal and one cytosine/adenine in the 5 & PRIME; terminal of CpG motifs that maintained the same resistance to degradation in serum as G2.0.0 and improved interleukin-6 production in RAW264 and bone marrow-derived macrophages. The immunostimulatory activity of antiparallel G4 CpG ODNs is superior to that of linear natural CpG ODNs. These results provide insights for the rational design of highly potent CpG ODNs using antiparallel G4 as a robust scaffold. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/83822 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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