| Title: | Effects of growth hormone and cortisol administration on plasma insulin-like growth factor binding proteins in juveniles of three subspecies of masu salmon (Oncorhynchus masou) |
| Authors: | Yamaguchi, Ginnosuke Browse this author |
| Habara, Shiori Browse this author |
| Suzuki, Shotaro Browse this author |
| Ugachi, Yuki Browse this author |
| Kawai, Hisashi Browse this author |
| Nakajima, Takuro Browse this author |
| Shimizu, Munetaka Browse this author →KAKEN DB |
| Keywords: | Insulin-like growth factor binding protein subtypes |
| Hormonal control |
| Growth hormone action |
| Ligand blotting |
| Acute stress |
| Oncorhynchus masou subspecies-complex |
| Issue Date: | Jan-2021 |
| Publisher: | Elsevier |
| Journal Title: | Comparative Biochemistry and Physiology Part A : molecular & integrative physiology |
| Volume: | 251 |
| Start Page: | 110821 |
| Publisher DOI: | 10.1016/j.cbpa.2020.110821 |
| Abstract: | In this study, we examined the effects of porcine growth hormone (GH) and cortisol on plasma insulin-like growth factor binding proteins (IGFBPs) in juveniles of three subspecies of Oncorhynchus masou (masu, amago, and Biwa salmon). Ligand blotting using digoxigenin-labeled human IGF-I was used to detect and semi-quantify three major circulating IGFBP bands at 41, 28, and 22 kDa, corresponding to IGFBP-2b, -1a, and -1b, respectively. GH increased plasma IGFBP-2b concentration in masu and Biwa salmon but suppressed it in amago salmon. Plasma IGFBP-2b levels were increased by cortisol in the three subspecies. Cortisol induced plasma IGFBP-1a in the three subspecies, whereas GH had a suppressive effect in masu and Biwa salmon. Sham and cortisol injections increased plasma IGFBP-1b levels after 1 day in masu and amago salmon, suggesting that IGFBP-1b is induced following exposure to stressors via cortisol. Increased IGFBP-1b levels were restored to basal levels when co-injected with GH in Biwa salmon, and the same trend was seen in masu and amago salmon. However, the suppressive effect of GH disappeared 2 days after injection in the three subspecies. Despite some differences among subspecies, the findings suggest that cortisol is a primary inducer of plasma IGFBP-1b; however, GH counteracts it in the short term. Therefore, GH has the potential to modulate the degree of increase in circulating IGFBP-1b levels during acute stress. |
| Rights: | © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license |
| http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/83823 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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