|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Competitive growth kinetics of Campylobacter jejuni, Escherichia coli O157: H7 and Listeria monocytogenes with enteric microflora in a small-intestine model
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
| Title: | Competitive growth kinetics of Campylobacter jejuni, Escherichia coli O157: H7 and Listeria monocytogenes with enteric microflora in a small-intestine model |
| Authors: | Fuchisawa, Yuto Browse this author | | Abe, Hiroki Browse this author | | Koyama, Kento Browse this author →KAKEN DB | | Koseki, Shigenobu Browse this author →KAKEN DB |
| Keywords: | bacterial competition | | Campylobacter jejuni | | dose-response | | enteric bacteria | | Escherichia coli O157 | | H7 | | in vitro intestinal model | | Listeria monocytogenes | | quantitative microbial risk assessment |
| Issue Date: | 23-Sep-2021 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Journal of applied microbiology |
| Volume: | 132 |
| Issue: | 2 |
| Start Page: | 1467 |
| End Page: | 1478 |
| Publisher DOI: | 10.1111/jam.15294 |
| Abstract: | Aims The biological events occurring during human digestion help to understand the mechanisms underlying the dose-response relationships of enteric bacterial pathogens. To better understand these events, we investigated the growth and reduction behaviour of bacterial pathogens in an in vitro model simulating the environment of the small intestine. Methods and Results The foodborne pathogens Campylobacter jejuni, Listeria monocytogenes and Escherichia coli O157:H7 were cultured with multiple competing enteric bacteria. Differences in the pathogen's growth kinetics due to the relative amount of competing enteric bacteria were investigated. These growth differences were described using a mathematical model based on Bayesian inference. When pathogenic and enteric bacteria were inoculated at 1 log CFU per ml and 9 log CFU per ml, respectively, L. monocytogenes was inactivated over time, while C. jejuni and E. coli O157:H7 survived without multiplying. However, as pathogen inocula were increased, its inhibition by enteric bacteria also decreased. Conclusions Although the growth of pathogenic species was inhibited by enteric bacteria, the pathogens still survived. Significance and Impact of the Study Competition experiments in a small-intestine model have enhanced understanding of the infection risk in the intestine and provide insights for evaluating dose-response relationships. |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/83900 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
