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Hokkaido University Collection of Scholarly and Academic Papers >
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In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens
Title: | In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens |
Authors: | Ariizumi, Takuma Browse this author | Murata, Shiro Browse this author →KAKEN DB | Fujisawa, Sotaro Browse this author | Isezaki, Masayoshi Browse this author | Sato, Takumi Browse this author | Oishi, Eiji Browse this author | Taneno, Akira Browse this author | Ichii, Osamu Browse this author →KAKEN DB | Maekawa, Naoya Browse this author →KAKEN DB | Okagawa, Tomohiro Browse this author →KAKEN DB | Konnai, Satoru Browse this author →KAKEN DB | Ohashi, Kazuhiko Browse this author →KAKEN DB |
Keywords: | poultry red mite | cysteine protease | vaccine candidate | Deg-CPR-2 | Dermanyssus gallinae |
Issue Date: | Mar-2022 |
Publisher: | Elsevier |
Journal Title: | Poultry Science |
Volume: | 101 |
Issue: | 3 |
Start Page: | 101638 |
Publisher DOI: | 10.1016/j.psj.2021.101638 |
Abstract: | Poultry red mites (PRMs, Dermanyssus gallinae) are hematophagous ectoparasites that negatively affect egg production, which causes serious economic losses to the poultry industry worldwide. Currently, the emergence of acaricide-resistant PRMs has impeded PRM control in poultry farms. Several alternatives for acaricide use have been described for managing PRM-caused problems. Vaccination is among the methods for controlling PRMs in poultry houses. Currently, several candidates for vaccine antigens have been identified. This study identified a cysteine protease, Deg-CPR-2, which differs from 2 other previously reported cysteine proteases in PRMs, from previously obtained data from RNA-sequencing (RNA-seq) analysis. We investigated the characteristics of Deg-CPR-2 and assessed its efficacy as a vaccine antigen in vitro. Phylogenetic analysis revealed that Deg-CPR-2 belonged to a different cluster from those of other cysteine proteases in PRMs. This cluster also included cathepsin L-like proteases, enzymes thought to be involved in hemoglobin digestion in ticks. Expression analysis revealed Deg-CPR-2 expression in midguts and all the life-stages; however, there were differences in the expression levels across the life-stages. The enzyme activity of recombinant Deg-CPR-2 was inhibited in the presence of a cysteine protease inhibitor, which suggests that Deg-CPR2 functions as a cysteine protease in PRMs. Finally, there was an in vitro increase in the mortality of PRMs, mainly protonymphs that were artificially fed with plasma from chickens immunized with Deg-CPR-2. These findings suggest that Deg-CPR-2 may contribute to protein digestion in the midgut of PRMs and is crucially involved in physiological processes in PRMs. Additionally, immunization with Deg-CPR-2 may reduce the number of protonymphs, and Deg-CPR-2 should be considered as a candidate antigen for anti-PRM vaccine development. |
Type: | article |
URI: | http://hdl.handle.net/2115/84051 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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